Thermo Scientific™ Pierce™ Traut's Reagent (2-iminothiolane), 500mg

Thermo Scientific™ Pierce™ Traut's Reagent, also known as 2-iminothiolane (2-IT), is a thiolation reagent that enables efficient and selective conversion of primary amines (such as those on lysine residues) into sulfhydryl (—SH) groups. The resulting thiolated biomolecules are then amenable to downstream conjugation via disulfide bonding, maleimide labeling, or immobilization on thiol-reactive supports.

This reagent features a short, five-atom spacer arm (8.1 Å) and is particularly useful in maintaining the native charge properties of biomolecules due to its preservation of a positive charge post-modification. The resulting free thiol groups are reactive under mild conditions, enabling controlled, site-specific crosslinking or labeling in a variety of bioconjugation applications.

Key Features & Benefits

• Single-Step Thiolation
  – Converts primary amines to sulfhydryl groups under mild conditions (pH 7–10).
• Maintains Positive Charge
  – Unique structure preserves the net positive charge of proteins, aiding solubility and native conformation.
• Minimal Steric Hindrance
  – Small molecular size and short spacer arm allow proximity-based applications and reduced structural disruption.
• Efficient & Selective
  – Selectively targets lysine residues and other primary amines without modifying carboxyl groups or hydroxyls.
• Highly Soluble in Organic Solvents
  – Dissolves readily in DMSO or DMF, making it compatible with aqueous or mixed-phase labeling protocols.

Applications

• Antibody Thiolation
  – Introduce reactive thiol groups for downstream maleimide conjugation or gold nanoparticle coupling.
• Protein Crosslinking
  – Prepare sulfhydryl-functionalized proteins for disulfide or heterobifunctional crosslinker reactions.
• Labeling & Detection
  – Enables conjugation of proteins to fluorescent dyes, biotin, enzymes, or beads via thiol-reactive chemistries.
• Surface Immobilization
  – Attach thiolated proteins or peptides to thiol-reactive resins or sensors (e.g., gold surfaces or activated beads).

Chemical & Physical Properties

Thermo Scientific™ Pierce™ Traut's Reagent is an essential tool for researchers requiring site-specific thiolation of amines while maintaining charge integrity and protein solubility. Its efficient, single-step chemistry and compatibility with a broad range of labeling and crosslinking protocols make it a versatile and valuable reagent in antibody engineering, protein conjugation, and biophysical assay development.