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Sigma-Aldrich™ Albumin, Bovine Serum (BSA), Fraction V, Fatty Acid-Free
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Specifications:
| Application | molecular biology, ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Powder |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Molecular Biology | ||
Sigma-Aldrich™ Bovine Serum Albumin (BSA), Fraction V, Fatty Acid-Free is a high-purity, widely used biochemical reagent designed for immunodiagnostics, cell culture, and molecular biology applications. Manufactured using the cold ethanol (Cohn) fractionation method, this BSA offers excellent consistency, low fatty acid content, and high binding capacity, making it ideal for sensitive assays and protein stabilization.
With a purity of ≥95% (cellulose acetate electrophoresis) and minimal impurities, this product is particularly suitable for applications requiring low background interference, such as ELISA, immunofluorescence, and binding studies.
Key Features
- High Purity (≥95%) – Ensures reliable and reproducible results
- Fatty Acid-Free (≤0.02%) – Minimizes interference in biochemical assays
- Fraction V Grade – Industry-standard for laboratory and diagnostic use
- Excellent Blocking Agent – Ideal for ELISA, immunoassays, and microscopy
- Protein Stabilization – Enhances stability of enzymes, antibodies, and cells
- Versatile Application Range – Suitable for molecular biology, immunology, and cell culture workflows
- Consistent Quality – Manufactured under strict quality control (Calbiochem® standard)
Applications
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Immunodiagnostics & ELISA
- Blocking buffer to reduce non-specific binding
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Cell Culture & Stem Cell Research
- Supports differentiation of human embryonic stem cells (hESC)
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Molecular Biology
- Stabilizer for enzymes and proteins
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Immunofluorescence & Immunoelectron Microscopy (IEM)
- Blocking agent for tissue staining
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Biochemical Assays
- Binding and transport studies for drugs, hormones, and fatty acids
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Mitochondrial Research
- Used in isolation buffers to trap free fatty acids
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Bovine Serum Albumin (BSA), Fraction V |
| Grade | Fatty Acid-Free |
| CAS Number | 9048-46-8 |
| Molecular Type | Non-glycosylated globular protein |
| Purity (Assay) | ≥95% (cellulose acetate electrophoresis) |
| Form | Powder |
| Fatty Acid Content | ≤0.02% |
| Ash Content | ≤3% |
| Moisture Content | ≤5% |
| Color | White to light yellow/light brown |
| pH | 6.8 – 7.4 |
| Manufacturing Method | Cold ethanol extraction (Cohn method) |
| Storage Temperature | 2–8°C |
| Shipping Conditions | Ambient |
| Storage Condition | Can be frozen |
| Stability (Reconstituted) | Up to 3 months at 4°C |
Available Pack Size
| Pack Size | SKU |
|---|---|
| 100 g | 126575-100GM |
Preparation & Handling
- Reconstitute in appropriate buffer or distilled water
- Allow up to 24 hours for complete dissolution
- Store reconstituted solution at 4°C
- Optional: Add 0.1% sodium azide (NaN₃) as preservative
- Follow standard laboratory safety procedures
Comparison with Standard BSA
| Feature | Fatty Acid-Free BSA | Standard BSA |
|---|---|---|
| Fatty Acid Content | ≤0.02% | Higher |
| Assay Sensitivity | High | Moderate |
| Background Interference | Minimal | Higher |
| Ideal Applications | ELISA, IEM, binding | General lab use |
Unique Advantages
- Ultra-low fatty acid content ensures accuracy in lipid-sensitive assays
- High structural stability due to 17 disulfide bonds
- Multi-functional protein carrier for drugs, hormones, and biomolecules
- Trusted Sigma-Aldrich / Calbiochem® quality for reproducible research
Sigma-Aldrich™ BSA, Fraction V, Fatty Acid-Free is a premium-grade protein reagent designed for high-sensitivity laboratory applications. Its exceptional purity, low impurity profile, and versatile functionality make it an essential component in immunoassays, cell culture systems, and advanced biochemical research workflows.