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Specifications:
| Application | qPCR, PCR, Reverse Transcription | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent, Dye | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ ROX Reference Dye is designed to provide a stable baseline for normalization of fluorescent reporter signals in real-time quantitative PCR (qPCR) and RT-qPCR assays. By compensating for well-to-well and instrument-related variations, it ensures improved reproducibility and accuracy of amplification data.
This dye has been qualified for use with ABI PRISM™ 7700, in combination with Platinum™ Quantitative PCR SuperMix-UDG™, Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System, and SuperScript™ III Platinum™ One-Step qRT-PCR Kit, making it a trusted choice for reliable multiplex qPCR workflows.
Key Features
- Signal Normalization – Corrects for non-PCR–related fluctuations in fluorescence intensity.
- Enhanced Accuracy – Provides a stable baseline for qPCR and RT-qPCR assays.
- Multiplex Ready – Supports accurate multiplex reactions by stabilizing fluorescent signals.
- Seamless Integration – Utilizes existing ROX spectral calibration, eliminating the need to re-calibrate thermal cyclers.
- Flexible Use – Compatible with multiple Invitrogen™ qPCR and RT-qPCR reagent systems.
Applications
- Quantitative PCR (qPCR) signal normalization
- RT-qPCR assays with multiplex detection
- Use with ABI PRISM™ 7700 and compatible systems
- Baseline fluorescence stabilization in real-time PCR
Technical Specifications
| Property | Details |
|---|---|
| Product Type | ROX Reference Dye |
| Quantity | 500 µL |
| Catalog Number | 12223012 |
| Detection Method | Primer-probe |
| Dye Label | ROX |
| PCR Method | qPCR, RT-qPCR |
| Unit Size | Each |
| Volume | 500 µL |
| Shipping Conditions | Wet or Dry Ice (approved) |
| Storage Temperature | –5 °C to –30 °C |
Contents & Storage
- Contents: ROX Reference Dye, supplied as a liquid solution.
- Storage: Store at –5 °C to –30 °C in a non-frost-free freezer to ensure long-term stability.
The Invitrogen™ ROX Reference Dye is an essential control reagent for real-time PCR and RT-PCR applications, ensuring fluorescence signal stability and reproducibility across wells and instruments. With proven compatibility in Invitrogen™ qPCR systems and multiplex assays, it provides researchers with the confidence of consistent, high-quality results in quantitative gene expression and nucleic acid detection workflows.