0
Specifications:
| Application | PCR | ||
| Storage Temperature | -15°C to -25°C | ||
| Product Type | PCR Reagent, Enzymes | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Invitrogen™ ezDNase™ Enzyme is a highly specific, recombinant double-strand-specific DNase designed for rapid and efficient removal of contaminating genomic DNA (gDNA) from RNA samples. It cleaves only double-stranded DNA into 2–8 bp fragments, preserving the integrity of RNA, cDNA, primers, and single-stranded nucleic acids in the reaction.
It is heat-labile and can be inactivated at 55°C, making it ideal for workflows that require clean, DNA-free RNA for RT-qPCR, cDNA synthesis, and other RNA-based applications.
Key Features
✅ Efficient Genomic DNA Removal
- Rapidly degrades double-stranded genomic DNA without degrading RNA
- Ensures clean RNA input for reverse transcription reactions
✅ Highly Specific to dsDNA
-
No impact on:
- RNA templates
- cDNA
- Primers
- Enables use in RNA cleanup prior to RT-qPCR without risk to sensitive nucleic acids
✅ Heat-Labile for Easy Inactivation
- Deactivated by incubation at 55°C, avoiding the need for DNase removal steps
- Simplifies workflows and improves reproducibility
✅ Compatible with Invitrogen RT Reagents
- Recommended for use with SuperScript™ IV VILO™ Master Mix and other Invitrogen reverse transcription kits
- Enhances cDNA synthesis accuracy by preventing gDNA-derived background amplification
Specifications
| Attribute | Details |
|---|---|
| Enzyme Type | Recombinant Double-Strand-Specific DNase |
| Concentration | 10X |
| Form | Liquid |
| Compatible Applications | RT-qPCR, cDNA synthesis, RNA preparation |
| Compatibility | SuperScript™ IV VILO™, other RT reagents |
| Sample Type | Total RNA |
| Unit Size | 50 reactions |
| Shipping Condition | Dry ice |
| Storage Temperature | –5°C to –30°C |
Contents & Storage
| Component | Volume |
|---|---|
| ezDNase Enzyme | 50 μL |
| ezDNase Buffer (10X) | 100 μL |
| Nuclease-free Water | 1.25 mL |
Store at –5°C to –30°C. Avoid repeated freeze-thaw cycles. Not for use in diagnostic procedures.
Use Cases
🧬 RT-qPCR and Gene Expression Studies
- Removes genomic DNA prior to reverse transcription to avoid false-positive signals in qPCR
🧪 cDNA Synthesis
- Ensures accurate cDNA generation by removing DNA contaminants that interfere with enzyme activity or primer binding
📦 RNA Sample Preparation for Downstream Applications
- Suitable for in vitro transcription, RNA-Seq library prep, or RNA integrity QC
Why Choose ezDNase™ Enzyme?
- ✅ Targeted gDNA removal without affecting other components
- ✅ Compatible with sensitive downstream applications
- ✅ Streamlined protocol with heat inactivation—no cleanup required
- ✅ Ideal for high-throughput and time-sensitive qPCR workflows