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SPINeasy® Host Depletion Microbial DNA Kit, 50 Preps
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Specifications:
| Application | Nucleic Acid Extraction | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Sample Prep | Forms | Liquid |
| Product Brand | MP Biologicals | ||
| Product Grade | Molecular Biology | ||
The SPINeasy Host Depletion Microbial DNA Kit is a specialized tool designed to isolate high-quality microbial DNA from samples with significant host DNA contamination. Using a combination of selective lysis, enzymatic degradation, and advanced purification techniques, this kit minimizes host DNA and maximizes microbial DNA recovery for downstream molecular applications such as microbiome analysis and next-generation sequencing (NGS).
Key Features
- Effective Host DNA Depletion:
- Selective lysis of host cells using a proprietary Host Lysis Buffer.
- Enzymatic degradation of residual host DNA and DNA from dead microbial cells.
- High Microbial DNA Recovery:
- Combines specialized buffers and mechanical homogenization for efficient microbial cell lysis.
- Delivers over 90% recovery of microbial DNA, validated through qPCR analyses.
- Convenient Workflow:
- Silica-membrane spin-column technology for easy and efficient DNA purification.
- Compatible with FastPrep instruments from MP Biomedicals for optimal homogenization.
- Validated Performance:
- Proven efficacy with saliva samples, demonstrating substantial host DNA depletion while preserving microbial DNA.
- Versatile Sample Compatibility:
- Suitable for saliva, swabs, and bodily fluids.
- Applications:
- Microbiome analysis
- Shotgun metagenomics sequencing
- Next-generation sequencing (NGS)
- Genomics and molecular biology research
Kit Contents
| Component | Volume/Quantity |
|---|---|
| Equilibration Buffer | 12 mL |
| Host Lysis Buffer | 60 mL |
| Host Depletion Buffer | 6 mL |
| Host Depletion Enzyme (HDE) | 1 vial |
| HDE Reconstitution Buffer | 200 µL |
| Microbial Selection Buffer | 12 mL |
| Microbial Lysis Buffer | 40 mL |
| Wash Buffer HD1 | 30 mL |
| Wash Buffer HD2 | 6 mL |
| Wash Buffer HD | 6 mL |
| Lysing Matrix E (50x) | Included |
| Spin Columns with Collection Tubes | 50x |
Specifications
| Specification | Details |
|---|---|
| Product Name | SPINeasy Host Depletion Microbial DNA Kit |
| SKU | 116545050 |
| Pack Size | 50 Preps |
| Storage Conditions | Room Temperature (15–25°C) |
| Sample Types | Saliva, swabs, bodily fluids |
| Alternate Names | Host DNA Depletion Kit |
| Product Line | SPINeasy |
Applications
- Microbiome Analysis:
- Ideal for investigating microbial communities with minimal host DNA interference.
- Shotgun Metagenomics:
- Enhances microbial DNA extraction for high-resolution metagenomic studies.
- Next-Generation Sequencing (NGS):
- Optimized for sequencing workflows requiring high-quality microbial DNA.
- Genomics and Molecular Biology:
- Supports diverse molecular analyses and genomic studies.
Performance Validation
- Host DNA Depletion Efficiency: Demonstrated by the significant reduction of host DNA using human β-globin primers.
- Microbial DNA Recovery: Achieves >90% recovery as shown through 16S primer-based qPCR analysis.
Care and Handling
- Store kit components at room temperature (15–25°C) in a dry environment.
- Follow provided protocols for optimal host depletion and microbial DNA isolation.
Why Choose SPINeasy?
- High Efficacy: Achieves exceptional host DNA removal while preserving microbial DNA integrity.
- Ease of Use: Simplified workflow with ready-to-use buffers and spin-column technology.
- Validated Performance: Proven results in challenging sample types such as saliva and bodily fluids.
- Versatile Applications: Tailored for microbiome research, metagenomics, and advanced molecular biology.
The SPINeasy Host Depletion Microbial DNA Kit is a reliable, efficient, and user-friendly solution for researchers working with host-contaminated samples, enabling robust microbial DNA extraction for advanced molecular and sequencing applications.