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Invitrogen™ Dynabeads™ Untouched™ Human T Cells Kit for 100 preps
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Specifications:
| Application | T Cell Research |
| Storage Temperature | 2-8°C |
| Product Type | Molecular Biology Reagent |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Molecular Biology |
The Dynabeads™ Untouched™ Human T-Cells Isolation Kit provides a highly efficient method for isolating pure and viable T-cells from peripheral blood mononuclear cells (PBMC) via negative isolation. This kit is designed to yield untouched T-cells with excellent purity, recovery, and viability, ideal for downstream functional assays and applications.
Key Features
- Untouched T-Cells:
- T-cells are isolated without direct contact with beads or antibodies, maintaining their natural state and functionality.
- High Purity and Viability:
- Produces T-cells with >95% viability and high recovery rates, suitable for sensitive applications.
- Simple and Scalable Workflow:
- No columns are required, enabling easy scalability for larger sample volumes.
- Wide Application Compatibility:
- Isolated T-cells are suitable for flow cytometry, functional assays, and other downstream applications.
- Fast and Efficient:
- Negative isolation eliminates non-T-cells quickly using Dynabeads™ and magnetic separation.
Specifications
| Feature | Details |
|---|---|
| Cell Type | T Cells (Whole Population) |
| Isolation Methodology | Negative Isolation |
| Sample Type | PBMC |
| Output Viability | >95% |
| Purity | High-purity, bead-free T-cells |
| Starting Material Cell No. | 1 x 10⁷ PBMCs per isolation |
| Final Product Type | Pure, viable, untouched T-cells |
| Technology | Dynabeads™ Magnetic Separation |
| No. of Tests | 100 isolations |
| Target Species | Human |
| Product Line | Dynabeads™, Untouched™, DYNAL™ |
| Storage Conditions | 2°C to 8°C |
| Shipping Condition | Room Temperature |
Methodology
- Preparation:
- Add the antibody mix (containing monoclonal antibodies against non-T-cells) to the PBMC sample.
- Binding:
- The antibodies bind specifically to non-T-cells (e.g., B cells, NK cells, monocytes, dendritic cells, granulocytes, erythrocytes).
- Bead Addition:
- Add Depletion Dynabeads™, which bind to the labeled non-T-cells.
- Magnetic Separation:
- Use a magnet to separate bead-bound non-T-cells from the untouched T-cells.
- Isolation:
- Discard the bead-bound non-T-cells. The remaining untouched T-cells are ready for use.
Contents
- Depletion Dynabeads™: 10 mL
- Antibody Mix: 2 mL, including monoclonal antibodies targeting:
- CD14, CD16a/b, CD19, CD36, CD56, CD123, CD235a (Glycophorin A)
Applications
- Flow Cytometry Analysis:
- Directly analyze isolated T-cells for phenotypic and functional studies.
- Functional Assays:
- Perform functional assays such as proliferation, activation, or cytokine secretion studies.
- Immunological Research:
- Investigate T-cell-mediated immunity and response.
- Gene Expression Studies:
- Extract high-quality RNA or DNA from untouched T-cells for transcriptional or genomic analysis.
Advantages
| Feature | Benefit |
|---|---|
| Negative Isolation | Yields pure, functional T-cells without antibody or bead contact. |
| High Viability | >95% viable T-cells, ideal for sensitive downstream applications. |
| No Columns Required | Simplifies workflow and reduces processing time. |
| Scalable Protocol | Adaptable for various sample sizes and experimental needs. |
| Wide Compatibility | Suitable for multiple assays, including flow cytometry and functional testing. |
Comparison with Competitor Kits
| Feature | Dynabeads™ Untouched™ T-Cells Kit | Miltenyi Biotec T-Cell Isolation Kit | STEMCELL EasySep™ T-Cell Kit |
|---|---|---|---|
| Isolation Method | Negative Isolation | Negative Isolation | Negative Isolation |
| Output Purity | High-purity, untouched T-cells | High-purity, untouched T-cells | High-purity, untouched T-cells |
| Viability | >95% | >95% | >95% |
| Workflow | No columns required, magnetic separation | Columns required | No columns required |
| Starting Material | PBMC | PBMC | PBMC |
| Number of Isolations | 100 | 50 | 100 |
| Storage | 2°C to 8°C | 2°C to 8°C | 2°C to 8°C |
| Ease of Use | High | Medium (column-dependent) | High |
| Best Feature | Truly untouched, bead-free T-cells | High recovery rates | No column-based processing |