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VWR™ Acrylamide/Bis-acrylamide Acryl/Bis™ 37.5:1 Solution
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Ready-to-use solutions for customized polyacrylamide gel electrophoresis of proteins and PAGE of nucleic acids.
Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | VWR | ||
| Product Grade | Molecular Biology | ||
Ultra-Pure, Ready-to-Use Monomer Solution for High-Resolution Polyacrylamide Gel Electrophoresis (PAGE)
The VWR™ Acrylamide/Bis-acrylamide Acryl/Bis™ 37.5:1 Solution is a pre-mixed, ready-to-use reagent for preparing polyacrylamide gels used in protein and nucleic acid electrophoresis. This formulation combines ultra-pure acrylamide and bis-acrylamide powders (>99.9%) in a 37.5:1 ratio, optimized for resolving proteins with high resolution and sharp band definition in SDS-PAGE and native PAGE workflows.
Designed to minimize exposure to neurotoxic acrylamide powders, this aqueous liquid solution offers increased safety, reproducibility, and convenience in laboratory gel casting procedures.
Key Features & Benefits
- 37.5:1 Acrylamide:Bis-acrylamide Ratio – Ideal for resolving medium to high molecular weight proteins
- Ultra-Pure Grade – >99.9% purity for reproducible gel polymerization and resolution
- Ready-to-Use Liquid Format – No need for weighing or handling powdered acrylamide
- Minimized Risk – Reduces exposure to hazardous neurotoxins in powdered acrylamide
- Flexible Use – Compatible with SDS-PAGE, native PAGE, and nucleic acid PAGE applications
Applications
- SDS-PAGE and Native PAGE – Protein electrophoresis for molecular weight separation
- DNA/RNA Electrophoresis – Polyacrylamide gels for high-resolution nucleic acid analysis
- 2D Electrophoresis – Crosslinking matrix for IEF-PAGE combinations
- Western Blotting & Proteomics – Downstream applications involving resolved protein samples
Technical Specifications
| Attribute | Specification |
|---|---|
| Product Name | Acryl/Bis™ 37.5:1 Solution |
| Supplier | VWR™ |
| Acrylamide:Bis Ratio | 37.5:1 |
| Form | Liquid (aqueous solution) |
| Purity | >99.9% acrylamide and bis-acrylamide powders |
| Product Type | Monomer solution for PAGE gel preparation |
| Use | Protein and nucleic acid electrophoresis |
| Safety Benefits | Reduces direct handling of powdered acrylamide |
| Storage Conditions | Store at 2–8°C; protect from light and contamination |
| Regulatory Use | For Research Use Only |
The VWR™ Acryl/Bis™ 37.5:1 Solution delivers high purity and ease of use for the preparation of reliable and reproducible polyacrylamide gels. This solution is an essential tool for researchers requiring safe, high-resolution electrophoresis for proteins and nucleic acids in molecular biology, proteomics, and biochemical analysis.