Invitrogen™ eBioscience™ Permeabilization Buffer (10X), 100 mL

For High-Performance Intracellular Staining in Flow Cytometry

The Invitrogen™ eBioscience™ Permeabilization Buffer (10X) is a key reagent for flow cytometric analysis of intracellular antigens, including cytokines, transcription factors, and nuclear proteins. This buffer is used after fixation to gently permeabilize cell membranes, enabling fluorochrome-conjugated antibodies to access intracellular targets.

Optimized to reduce nonspecific binding and increase signal-to-noise ratio, this buffer is compatible with IC Fixation Buffer (Cat. No. 00-8222) and the Foxp3 Fixation/Permeabilization Concentrate and Diluent (Cat. No. 00-5521-00).

Key Features and Benefits

• Optimized for Intracellular Staining
  Enhances antibody penetration and reduces background staining, improving detection sensitivity.
• Compatible with Transcription Factor Detection
  Ideal for staining Foxp3, Ki-67, cytokines, and other nuclear or cytoplasmic markers.
• Supports High-Quality Flow Cytometry Results
  Maintains fluorochrome stability while providing reliable cell integrity post-fixation.
• Flexible Use Across Protocols
  Use for washing, antibody dilution, and cell resuspension after permeabilization.

Applications

• Intracellular cytokine staining (ICS)
• Transcription factor analysis (e.g., Foxp3, Ki-67)
• Immune cell subset analysis
• Multiparameter flow cytometry staining protocols

Technical Specifications

Invitrogen™ eBioscience™ Permeabilization Buffer (10X) is a trusted reagent for intracellular flow cytometry applications, enabling efficient and reproducible staining of cytoplasmic and nuclear proteins. It is a critical component of any intracellular staining protocol where antibody specificity, fluorochrome performance, and sample quality are essential.