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Varioskan™ ALF Multimode Microplate Reader, for 6- to 384-well microplates
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Measurement Modes: Absorbance, Fluorescence, Luminescence, Turbidimetry
Specifications:
| Application | ELISA-Based Assays, Fluorescence-Based Assay | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Varioskan™ ALF Multimode Microplate Reader is a high-performance, entry-level multimode reader designed for most common absorbance, turbidimetric, fluorescence, and luminescence assays.
Its xenon flash lamp and monochromator-based photometric design provide continuous wavelength selection from 200 nm to 1000 nm, supporting a broad range of life science and drug discovery applications — including ELISA, nucleic acid quantification, protein assays, bacterial growth curves, and cell viability measurements.
Featuring filter-based optics for fluorescence and luminescence, the Varioskan™ ALF delivers enhanced specificity, sensitivity, and signal stability, making it an ideal choice for laboratories performing routine and advanced plate-based analyses.
Key Features
1. Multimode Flexibility
- Measures absorbance, fluorescence, luminescence, and turbidity in a single platform.
- Photometric mode: Monochromator-based continuous wavelength selection from 200–1000 nm.
- Filter-based optics for fluorescence and luminescence for optimal signal-to-noise performance.
2. Enhanced Optical Performance
- Xenon flash lamp for high-intensity, broadband excitation.
- Three pre-installed fluorescence filter sets for coumarin, fluorescein, and resorufin families.
- Five additional open filter slots for customizable assays (e.g., FRET, BRET, or custom fluorophores).
3. Intelligent Measurement Control
- Automatic gain adjustment ensures consistent signal detection and eliminates manual parameter optimization.
- Dynamic range ≥6 decades for fluorescence and luminescence.
- Fast measurement modes: Photometry ≤10 s; Fluorescence/Luminescence ≤20 s (96-well).
4. Intuitive Operation and Compatibility
- Controlled via Thermo Scientific™ SkanIt™ Software (Research or Drug Discovery Edition).
- No annual license fee and unlimited installations.
- Compatible with 6- to 384-well plates and μDrop plates for microvolume DNA, RNA, and protein quantification.
- Performs endpoint, kinetic, spectral scanning, and turbidimetric measurements.
5. Built-In Incubation and Shaking
- Integrated incubation system maintains temperatures from ambient +2°C to 45°C.
- Supports linear, orbital, and double-orbital shaking modes for uniform well mixing.
6. Data Integrity and Automation
- Onboard self-diagnostics for operational reliability.
- Compatible with laboratory automation systems through standard interface ports.
- Verification plates available for traceable optical validation with pre-defined SkanIt™ protocols.
Technical Specifications
| Parameter | Specification |
|---|---|
| Measurement Modes | Absorbance, Fluorescence, Luminescence, Turbidimetry |
| Wavelength Range (Absorbance) | 200–1000 nm |
| Excitation/Emission Range | Excitation: 200–710 nm |
| Photometric Bandwidth | 2.5 nm |
| Photometric Accuracy | ±1.0% + 0.003 Abs (0–2 Abs); ±2.0% (2.0–2.5 Abs) at 450 nm |
| Dynamic Range | ≥6 decades (fluorescence/luminescence) |
| Photometric Sensitivity | DNA <1 ng/µL (96-well half-area plate) |
| Fluorescence Sensitivity | 0.35 fmol fluorescein/well (black 384-well plate) |
| Luminescence Sensitivity | <275 amol ATP/well (white 96-well plate) |
| Turbidimetry Sensitivity | <5 FTU (formazin) |
| Plate Compatibility | 6- to 384-well microplates; μDrop and μDrop Duo plates |
| Incubation Range | Ambient +2°C to 45°C |
| Reading Options | Endpoint, kinetic |
| Shaking Modes | Linear, orbital, double-orbital |
| Spectral Scanning Speed | ≤16 s (fast mode) |
| Light Source | Xenon flash lamp |
| Detector | Photomultiplier tube (PMT) |
| Interface | PC via SkanIt™ Software |
| Software Languages | English, French, German, Spanish, Portuguese, Italian, Japanese, Chinese |
| Voltage | 100/115/220/230 V |
| Dimensions (L × W × H) | 61 × 34 × 40 cm |
| Weight | 24.5 kg |
| Warranty | 1-year standard (2-year Rapid Exchange available) |
Applications
- Quantification: DNA, RNA, and protein concentration assays.
- Cell-Based Assays: PrestoBlue™, AlamarBlue™, and viability tests.
- Enzyme Kinetics and ELISA: High-accuracy absorbance and luminescence detection.
- Microbial Studies: Bacterial growth and turbidimetric assays.
- Drug Discovery: FRET, BRET, and compound screening.
- Reporter Gene Studies: Luciferase, GFP, and β-galactosidase quantification.
Included Components
- Varioskan™ ALF Multimode Reader (Cat. No. VA000010C)
- Thermo Scientific™ SkanIt™ Software – Research Edition
- Power cable and communication interface
- User manual and quick-start guide
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Factory-installed filters:
- Coumarin (Ex 345/25 nm; Em 450/40 nm)
- Fluorescein (Ex 485/15 nm; Em 525/15 nm)
- Resorufin (Ex 555/15 nm; Em 615/45 nm)
The Thermo Scientific™ Varioskan™ ALF Multimode Microplate Reader is a robust, flexible, and precise instrument for absorbance, fluorescence, and luminescence assays.
With filter-based sensitivity, monochromator-based photometry, automatic gain adjustment, and SkanIt™ Software integration, it offers superior data quality and reproducibility for a wide range of molecular, biochemical, and cellular assays.
Ideal for life sciences, drug discovery, and academic laboratories, the Varioskan™ ALF combines performance, ease of use, and expandability — backed by Thermo Scientific reliability.