Thermo Scientific™ Penicillin G sodium salt

Thermo Scientific™ Penicillin G Sodium Salt is a high-purity beta-lactam antibiotic widely used in microbiology and cell culture for its antimicrobial properties. It is effective against Gram-positive organisms and certain Gram-negative organisms due to its ability to inhibit bacterial cell wall synthesis by targeting penicillin-binding proteins (PBPs).

Key Features

1. Composition:
   • Active Ingredient: Penicillin G sodium salt (benzylpenicillin sodium).
   • Molecular Weight: 356.38 g/mol.
   • CAS Number: 69-57-8.
   • Chemical Formula: C₁₆H₁₇N₂NaO₄S.
2. Form:
   • Fine, white to off-white powder.
   • Water-soluble.
3. Applications:
   • Antimicrobial agent in microbial culture media.
   • Antibiotic in cell culture applications to prevent bacterial contamination.
   • Used in susceptibility testing for determining bacterial resistance.
4. Mechanism of Action:
   • Inhibits bacterial cell wall synthesis by interfering with the cross-linking of peptidoglycan, an essential component of the bacterial cell wall.
   • Active against Gram-positive organisms like Staphylococcus aureus, Streptococcus pneumoniae, and Gram-negative organisms like Neisseria gonorrhoeae.
5. Standards and Certifications:
   • Complies with USP and EP quality standards.
   • Suitable for laboratory and clinical use.
6. Storage:
   • Store at 2–8°C in a dry environment.
   • For long-term storage, keep at -20°C to maintain stability.

Applications in Microbiology and Cell Culture

1. Microbial Culture Media:
   • Penicillin G sodium salt is commonly added to growth media to prevent bacterial contamination in laboratory and industrial fermentation processes.
2. Susceptibility Testing:
   • Utilized in antimicrobial susceptibility testing (AST) for determining resistance profiles of clinical isolates in accordance with EUCAST and CLSI standards.
3. Cell Culture:
   • Penicillin is often used in combination with streptomycin in cell culture media as a broad-spectrum antimicrobial agent.

Specifications

Quality Control Data

Comparison with Similar Products

Test Procedure

1. Preparation of Stock Solution:
   • Dissolve the required amount of Penicillin G sodium salt in sterile water or buffer to prepare a stock solution.
2. Dilution for Use:
   • Prepare working concentrations depending on the specific assay or media requirements.
3. Application:
   • Add directly to media or use as part of a combination for cell culture contamination prevention.
4. Quality Control:
   • Test against control strains to confirm potency and expected MIC values.

Thermo Scientific™ Penicillin G Sodium Salt offers unmatched reliability for microbial and cell culture applications, adhering to stringent quality standards, making it an essential component in laboratories worldwide.