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Thermo Scientific™ Sudan IV
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Lipid-Soluble Dye for Staining Neutral Fats, Waxes, and Lipophilic Compounds
Specifications:
| Application | Microbiology | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Dye | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Analytical grade | Formula | C₂₄H₂₀N₄O |
Thermo Scientific™ Sudan IV is a lipid-soluble diazo dye widely used in histology, cytology, and biochemical assays for the detection and visualization of neutral lipids, triglycerides, lipoproteins, and fat-soluble vitamins.
This reagent produces a distinct red coloration in lipid-rich regions, making it suitable for tissue staining, forensic analysis, and fat content evaluation in biological and industrial applications. Its high dye content and controlled purity ensure consistent staining intensity and reproducibility across experiments.
Chemical Information
| Property | Details |
|---|---|
| Chemical Name | Sudan IV |
| Synonyms | Oil Red, Scarlet Red, Fat Red B, Solvent Red 24, Oil Red IV, Lipid Crimson, 1-((2-Methyl-4-(o-tolyldiazenyl)phenyl)diazenyl)naphthalen-2-ol |
| Chemical Formula | C₂₄H₂₀N₄O |
| Molecular Weight | 380.45 g/mol |
| CAS Number | 85-83-6 |
| EINECS | 201-635-8 |
| MDL Number | MFCD00003893 |
| Merck Index | 14,8393 |
| Melting Point | ~185 °C (decomposes) |
| Storage Temperature | Ambient |
Physical and Chemical Properties
| Appearance | Deep red crystalline powder |
|---|---|
| Solubility | Soluble in ethanol, benzene, and chloroform; insoluble in water |
| Usage | Lipid staining dye (neutral fats, waxes, lipoproteins) |
| Hazard Classification | Irritant (Xi) |
Applications
- Histological and cytological staining for lipids in frozen tissue sections.
- Visualization of fat droplets in adipocytes and lipid-laden macrophages.
- Qualitative analysis of neutral fats and phospholipids in cells and tissues.
- Used in biochemical assays for fat and oil content detection.
- Forensic and toxicological studies involving lipid distribution.
Specifications
| Test Parameter | Specification |
|---|---|
| Purity | Analytical grade |
| Melting Point | ~185 °C (decomposes) |
| Staining Capability | High contrast for neutral lipid detection |
| Grade | Research and analytical reagent |
Safety and Handling
⚠️ Hazard Statements:
- Causes skin and eye irritation.
- Avoid inhalation of dust.
- Use with adequate ventilation.
Precautions:
- Wear gloves, goggles, and protective clothing.
- Avoid ingestion and contact with mucous membranes.
- Dispose of waste in accordance with local environmental regulations.
📦 Hazard Classification: Irritant
- GHS Pictograms: ⚠️
- Recommended PPE: Laboratory gloves, protective eyewear, lab coat.
Packaging and Ordering Information
| Catalog Number | Packaging Type | Pack Size | Container |
|---|---|---|---|
| A12181.14 | Glass bottle for solids | 25 g | Acroseal glass container |
| A12181.22 | Glass bottle for solids | 100 g | Acroseal glass container |
Storage and Stability
- Store at ambient temperature, away from direct light and heat.
- Keep tightly closed in original glass container.
Thermo Scientific™ Sudan IV is a high-quality lipid stain that provides reliable, reproducible visualization of fats and lipids in biological and analytical applications. Its well-defined purity and consistent staining intensity make it an essential reagent for histology laboratories, research facilities, and industrial quality control testing.
- Pack Size: 100g 25g