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Specifications:
| Application | Cell Analysis, Apoptosis | ||
| Storage Temperature | -20°C | ||
| Product Type | Biochemical Reagent | Forms | Solid |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | Formula | C₁₆H₂₄O₄ |
Thermo Scientific™ Brefeldin A (≥98%) is a macrocyclic lactone antibiotic known for its potent inhibitory effect on protein transport and secretion in eukaryotic cells. It acts by disrupting Golgi apparatus function, specifically blocking vesicular trafficking between the endoplasmic reticulum (ER) and the Golgi complex.
Due to its ability to block protein export, Brefeldin A is widely used in cell biology, immunology, and cancer research, particularly in studying intracellular trafficking, apoptosis, and Golgi-mediated processes.
Key Features
- High Purity: ≥97.5% by HPLC.
- Biologically Active Antibiotic: Inhibits protein transport in mammalian and eukaryotic cells.
- Mechanism of Action: Disrupts Golgi apparatus function and blocks ER-to-Golgi transport.
- Apoptosis Induction: Used to study Brefeldin A–mediated apoptosis in tumor cell models.
- Versatile Solubility: Soluble in DMSO, ethanol, and dichloromethane; slightly soluble in water.
- Research Grade: For Research Use Only (RUO); not intended for diagnostic or therapeutic use.
- Legacy Alfa Aesar Product: Produced under Thermo Scientific Chemicals brand with consistent quality and purity.
Chemical Identifiers
| Property | Value |
|---|---|
| Chemical Name (IUPAC) | (1S,6S,11aS,13S,14aR)-1,13-dihydroxy-6-methyl-cyclopenta[f]oxacyclotridecan-4-one |
| Synonyms | Ascotoxin, Brefeldin A |
| CAS Number | 20350-15-6 |
| Molecular Formula | C₁₆H₂₄O₄ |
| Molecular Weight | 280.36 g/mol |
| InChI Key | KQNZDYYTLMIZCT-PNFJWZTBSA-N |
| SMILES | C[C@H]1CCC\C=C[C@@H]2CC@HC[C@H]2C@@H\C=C\C(=O)O1 |
Specifications
| Parameter | Specification |
|---|---|
| Purity (HPLC) | ≥97.5% |
| Form | Crystals or crystalline powder |
| Appearance (Color) | White to pale yellow |
| Solubility | Soluble in DMSO, dichloromethane, and ethanol; slightly soluble in water |
| Storage Conditions | Store in a cool, dry place; avoid strong oxidizing agents |
| Stability | Stable under recommended storage conditions |
| Intended Use | Research Use Only (RUO) |
Applications
- Cell Biology & Immunology: Inhibition of protein secretion and Golgi function.
- Cancer Research: Study of Brefeldin A–induced apoptosis in tumor cells.
- Intracellular Transport Studies: Mechanistic investigations of ER–Golgi vesicle trafficking.
- Molecular and Cell Signaling Research: Disruption of protein synthesis and secretory pathways.
- Lipid Metabolism Studies: Known to induce lipid storage in Chlamydomonas reinhardtii.
References
- Wase, N. et al. “Phenotypic screening identifies Brefeldin A as an inducer of lipid storage in Chlamydomonas reinhardtii.” Algal Research (2015) 11, 74–84.
- Fuchs, M. & Fürstner, A. “trans-Hydrogenation: Application to a concise synthesis of Brefeldin A.” Angew. Chem. (2015) 54(13), 3978–3982.
The Thermo Scientific™ Brefeldin A, 98% is a high-purity, biologically active antibiotic essential for investigating Golgi apparatus function, protein transport, and apoptosis.
Its consistent quality, verified purity, and reproducible biological performance make it ideal for advanced cell biology and biochemical research requiring precise inhibition of intracellular trafficking.
- Pack Size: 10mg 25mg 50 mg