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Thermo Scientific™ (4-Nitrophenyl)-β-D-glucopyranoside ≥98%
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Specifications:
| Application | Colorimetric Assay | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Analytical grade | Formula | C₁₂H₁₅NO₈ |
The Thermo Scientific™ (4-Nitrophenyl)-β-D-glucopyranoside (pNPG) is a high-purity chromogenic substrate widely used in enzymatic assays for β-glucosidase activity. Upon enzymatic hydrolysis, pNPG releases p-nitrophenol, producing a measurable yellow color that can be quantified spectrophotometrically, making it an essential reagent in biochemistry, enzymology, and molecular biology research.
Key Features
- High purity ≥98% for reliable analytical performance
- Standard substrate for β-glucosidase enzyme assays
- Produces quantifiable chromogenic signal (p-nitrophenol)
- Suitable for colorimetric enzyme activity measurement
- Stable under recommended storage conditions
- Supplied in convenient glass bottles (1 g and 5 g options)
Applications
This compound is ideal for:
- β-glucosidase activity assays
- Enzyme kinetics and inhibition studies
- Carbohydrate metabolism research
- Microbial enzyme screening
- Biotechnology and industrial enzyme analysis
- Glycosidase enzyme characterization
Principle of Use
pNPG acts as a synthetic substrate for β-glucosidase enzymes:
- The enzyme hydrolyzes pNPG
- p-nitrophenol (pNP) is released
- Under alkaline conditions, pNP produces a yellow color
- The intensity is measured at ~405 nm, proportional to enzyme activity
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | (4-Nitrophenyl)-β-D-glucopyranoside (pNPG) |
| CAS Number | 2492-87-7 |
| Molecular Formula | C₁₂H₁₅NO₈ |
| Molecular Weight | 301.25 g/mol |
| Purity | ≥98% |
| Form | Solid (Powder) |
| Melting Point | 166–167°C |
| Optical Rotation | -103° (c=1 in water) |
| Storage | 2–8°C or freezer |
| Packaging | Glass bottles (1 g, 5 g) |
Synonyms
- p-Nitrophenyl-β-D-Glucopyranoside
- pNPG
- Hydroxybrasilin derivative
- Chromogenic glucoside substrate
Advantages
- Reliable substrate for quantitative enzyme assays
- High sensitivity and reproducibility
- Compatible with standard spectrophotometric methods
- Ideal for both research and industrial enzyme applications
- High chemical stability and purity
The Thermo Scientific™ (4-Nitrophenyl)-β-D-glucopyranoside (pNPG) is a trusted and widely used reagent for β-glucosidase activity measurement and enzymatic studies. Its high purity, strong chromogenic response, and versatility make it an essential tool for biochemical research, enzyme screening, and analytical applications.
- Pack Size: 5 Grams 1 Gram