QIAcard™ FTA™ Wash Buffer Nucleic acid purification buffer

The QIAcard FTA Wash Buffer is a specialized reagent designed for the efficient purification of nucleic acids from QIAcard FTA Cards, Indicating FTA Cards, and EasiCollect formats. Engineered to support reliable DNA recovery from FTA matrices, this proprietary buffer ensures the removal of contaminants and PCR inhibitors, enabling high-quality nucleic acid preparation for sensitive downstream applications.

Optimized for forensic and molecular biology workflows, the buffer facilitates efficient DNA release from FTA card punches, making it ideal for STR analysis, PCR amplification, and DNA profiling. Its robust formulation ensures consistent performance across high-throughput laboratory environments.

Key Features

Optimized for FTA Card-Based DNA Extraction

• Specifically developed for use with QIAcard FTA, Indicating FTA, and EasiCollect cards
• Enables efficient DNA release from the FTA matrix
• Supports “punch-in” workflows for streamlined sample processing

Enhanced PCR Performance

• Effectively removes PCR inhibitors and contaminants
• Improves DNA purity and amplification efficiency
• Ensures consistent and reproducible PCR results

STR Analysis Ready

• Produces high-quality DNA suitable for Short Tandem Repeat (STR) analysis
• Ideal for forensic DNA profiling and identity testing workflows

High-Throughput Capability

• Available in 500 mL format for large-scale processing
• Supports multiple reactions in busy laboratory environments
• Also available in 25 mL format for smaller-scale applications

Reliable and Convenient

• Stable at room temperature for easy storage and handling
• Ready-to-use formulation minimizes preparation steps
• Designed for consistent performance across applications

Applications

• DNA extraction and purification from FTA cards
• Forensic DNA analysis and profiling
• STR amplification and genotyping
• PCR-based molecular biology workflows
• Genetic research and population studies
• Sample preparation for sequencing and diagnostics
• Biobanking and high-throughput genomic processing

Technical Specifications

Ordering Information

Workflow Integration

Sample Collection → FTA Card Storage → Punch Extraction → Wash (FTA Wash Buffer) → PCR Amplification → STR Analysis / Sequencing

This workflow ensures:

• Efficient DNA recovery from FTA matrices
• Removal of inhibitors prior to amplification
• Reliable downstream molecular analysis

Unique Advantages

• Ensures high-efficiency DNA recovery from FTA cards
• Significantly improves PCR yield and amplification success
• Validated for forensic and STR applications
• Supports both low- and high-throughput workflows
• Compatible with multiple FTA-based sample collection formats
• Easy-to-use, ready-to-apply reagent

Regulatory & Safety Information

• For Research Use Only (RUO) – Not intended for diagnostic or therapeutic use
• Handle according to standard laboratory safety protocols
• Refer to the Safety Data Sheet (SDS) for detailed handling and disposal guidelines

The QIAcard FTA Wash Buffer is a critical component in FTA-based DNA workflows, delivering reliable nucleic acid purification and enhanced PCR performance. Whether used in forensic laboratories, research institutions, or high-throughput genomic facilities, it ensures consistent, high-quality results—making it an indispensable reagent for modern molecular biology applications.