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Thermo Scientific™ FastDigest™ Hin1II, 100 μL (100 Reactions)
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Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ FastDigest Hin1II is a high-quality, fast-acting restriction endonuclease that recognizes the CATG^ sequence and cleaves DNA efficiently to produce 3′ overhangs. Engineered for use in FastDigest™ and FastDigest™ Green Buffers, this enzyme enables complete digestion in just 5–15 minutes at 37°C, with 100% activity across all FastDigest-compatible enzymes and buffers. The enzyme is ideal for high-throughput molecular workflows, including restriction mapping, SNP detection, and cloning.
Key Features & Benefits
- Fast and Efficient: Complete digestion in 5–15 minutes
- Universal Buffer Compatibility: No need for buffer exchange in single, double, or multiple digestions
- No Star Activity: Optimized formulation prevents off-target cleavage
- Gel-Ready Reaction Mix: FastDigest Green Buffer includes tracking dyes and density reagent
- Heat Inactivation: Simple inactivation at 80°C
- Stable Storage: Recommended at –70°C; 6-month half-life at –20°C
- Downstream Ready: Compatible with ligation, blunting, dephosphorylation, and other modifying enzymes directly in the same buffer
Applications
- Molecular Cloning – Fast fragment generation for cloning workflows
- Restriction Mapping – Digest DNA with high precision for mapping applications
- Genotyping & RFLP – Identify polymorphisms or SNPs in target DNA
- Southern Blotting – Prepare DNA for probe-based detection
- Multiplex Digestions – Use multiple FastDigest enzymes in one buffer without protocol adjustment
Specifications Table
| Attribute | Details |
|---|---|
| Product Name | FastDigest Hin1II |
| Catalog Number | FD1834 |
| Recognition Sequence | 5′–CATG↓–3′ / 3′–↑GTAC–5′ |
| Quantity | 100 μL (approx. 100 reactions) |
| Overhang Type | 3′ protruding ends |
| Reaction Time | 5–15 minutes |
| Optimal Temperature | 37°C |
| Inactivation Temperature | 80°C |
| Methylation Sensitivity | Not sensitive to Dam, Dcm, or CpG methylation |
| Buffers Included | 10X FastDigest Buffer (1 mL), 10X FastDigest Green Buffer (1 mL) |
| Storage Conditions | –20°C (–70°C recommended for extended stability) |
| Type IIS | No |
| Product Type | Restriction Enzyme |
| Intended Use | Research use only (non-diagnostic) |
Ordering Information
| Catalog Number | Description | Pack Size |
|---|---|---|
| FD1834 | FastDigest Hin1II, 100 μL | 100 Reactions |
Thermo Scientific™ FastDigest Hin1II offers a fast, convenient, and reliable solution for DNA cleavage at CATG sites. With complete digestion in minutes, compatibility with all FastDigest protocols, and no star activity, it’s an indispensable enzyme for high-throughput molecular biology applications.