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Thermo Scientific™ Pierce™ Protease Inhibitor XL Capsules, EDTA-free
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Specifications:
| Application | molecular biology, Protein Purification | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Tablets |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Pierce™ Protease Inhibitor XL Capsules, EDTA-free, are designed for efficient and reliable protection of proteins during large-volume cell lysis and protein extraction procedures. Each capsule contains a broad-spectrum formulation of protease inhibitors and is sufficient for preparing up to 500 mL of solution, making it ideal for high-throughput and scale-up applications.
These capsules provide a convenient, ready-to-use alternative to traditional inhibitors such as PMSF, offering enhanced protection across multiple protease classes while maintaining compatibility with downstream protein analysis workflows.
Key Features
Broad-Spectrum Protease Inhibition
- Inhibits serine, cysteine, aspartic proteases, and aminopeptidases
- Provides stronger and more comprehensive protection than PMSF alone
- Preserves protein integrity during extraction and processing
Large-Volume Format
- One capsule sufficient for 500 mL of buffer or lysate
- Ideal for bulk sample preparation and high-throughput workflows
- Reduces preparation time and reagent handling
EDTA-Free Formulation
- Suitable for applications involving metalloproteins and metal-dependent enzymes
- Prevents interference with metal-sensitive assays
Convenient and Fast Dissolution
- Capsule format allows easy handling and dosing
- Dissolves completely within approximately 3 minutes
- Produces a clear solution ready for immediate use
Compatible with Downstream Applications
- Compatible with Pierce protein assays
- Suitable for detergent-based lysis buffers
- Can be combined with phosphatase inhibitors if required
Applications
- Large-volume protein extraction from tissues and cultured cells
- High-throughput proteomics workflows
- Cell lysis and sample preparation
- Western blotting and immunoassays
- Protein purification and enzyme studies
Mechanism of Action
During cell lysis, endogenous proteases are released and can degrade target proteins. Pierce Protease Inhibitor XL Capsules contain a combination of inhibitors that act on different protease classes:
- AEBSF – inhibits serine proteases
- Bestatin – inhibits aminopeptidases
- E-64 – inhibits cysteine proteases
- Pepstatin A – inhibits aspartic proteases
This combination ensures broad-spectrum inhibition and effective protection of proteins during large-scale extraction processes.
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Type | Protease Inhibitor Cocktail |
| Format | Capsules (solid) |
| Quantity | 10 Capsules |
| Inhibitor Type | Broad-spectrum protease inhibitors |
| EDTA Content | EDTA-free |
| Sufficient For | 500 mL per capsule |
| Compatibility | Protein assays, detergent-based lysis buffers |
| Shipping Condition | Room temperature |
| Storage | 2°C to 8°C |
| Product Line | Pierce™ |
| Unit Size | Each |
Usage Instructions
- Twist open one capsule
- Add contents to 500 mL of buffer or lysate
- Mix until fully dissolved (approximately 3 minutes)
- Use immediately in protein extraction workflow
Product Information
| Catalog Number | Description | Quantity |
|---|---|---|
| A37989 | Pierce Protease Inhibitor XL Capsules, EDTA-free | 10 Capsules |
Storage and Stability
- Store at 2°C to 8°C
- Maintain in tightly sealed container
- Protect from moisture
Related Products
- Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
- Halt Phosphatase Inhibitor Cocktail, EDTA-free (100X)
- T-PER Tissue Protein Extraction Reagent
Thermo Scientific™ Pierce™ Protease Inhibitor XL Capsules, EDTA-free, offer a highly efficient and scalable solution for protecting proteins during large-volume extraction workflows. With broad-spectrum inhibition, convenient capsule format, and compatibility with downstream assays, they are an essential reagent for laboratories performing high-throughput proteomics and protein research.