0
Specifications:
| Application | neural stem cell culture | ||
| Storage Temperature | -20°C | ||
| Product Type | Supplement | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
The Gibco™ B-27™ Plus Supplement (50X) is the next-generation, serum-free neuronal culture supplement, building upon the performance of the classic B-27™ Supplement—the most cited neuronal cell culture product worldwide.
Optimized for use with Gibco™ Neurobasal™ Plus Medium, B-27™ Plus provides the highest in vitro survival rates for primary rodent neurons and human stem cell–derived neurons, enabling advanced neuronal maturation and network functionality.
Enhanced through an upgraded formulation, refined raw material control, and stringent manufacturing standards, B-27™ Plus delivers superior neuron viability, electrophysiological performance, and synaptic connectivity for demanding neurobiology applications.
Key Features
- Next-generation formulation: Improved over classic B-27™ for superior neuron survival and functional performance.
- Unmatched survival rates: Achieves ≥50% higher survival of primary rat neurons versus traditional systems.
- Enhanced activity and maturation: Promotes consistent, synchronized firing and robust synaptic activity after 10 days in vitro.
- Validated performance: Outperforms BrainPhys™ Neuronal Medium in electrophysiological stability and maturation rate.
- Versatile and compatible: For use with Neurobasal™ Plus Medium and other neuronal culture platforms.
- High manufacturing quality: Produced under cGMP and ISO 13485-certified standards for reproducibility.
- Research Use Only (RUO): Not for diagnostic or therapeutic use.
Specifications
| Parameter | Specification |
|---|---|
| Cell Type | Neural cells (primary or stem cell–derived) |
| Concentration | 50X |
| Culture Type | Mammalian cell culture |
| Product Line | B-27™ |
| Serum Level | Serum-free |
| Form | Liquid |
| Sterility | Sterile |
| Tested For | Endotoxin, performance, sterility |
| Quantity | 10 mL |
| Shelf Life | 12 months |
| Storage Conditions | −5 to −20 °C; protect from light |
| Shipping Conditions | Dry ice |
| Intended Use | Research Use Only (RUO) |
Applications
- Serum-free culture of primary neurons (rat, mouse, human).
- Long-term maintenance of neuronal cultures for electrophysiological assays.
- Differentiation of stem cell–derived neurons and organoid systems.
- Studies in synaptogenesis, neurite outgrowth, and network activity.
- Neurobiology and drug discovery research requiring high neuronal viability.
The Gibco™ B-27™ Plus Supplement (50X) represents a major advancement in serum-free neuronal culture systems.
When paired with Neurobasal™ Plus Medium, it delivers unmatched neuronal survival, activity, and maturation, enabling researchers to achieve consistent, high-quality results in CNS modeling, electrophysiology, and neurodevelopmental research.
Backed by Gibco’s cGMP manufacturing and stringent quality controls, B-27™ Plus is the next-generation standard for neuronal culture excellence.