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Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Culture Medium | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Gibco™ StemPro™ NSC Serum-Free Medium (SFM) is a specialized, complete culture system developed for the growth, maintenance, and expansion of human neural stem cells (NSCs). Designed to support consistent proliferation while preserving the cells’ multipotent differentiation potential, this serum-free medium enables researchers to generate high yields of NSCs across multiple passages.
StemPro™ NSC SFM is supplied as a complete kit, eliminating the need for additional supplementation. The system combines KnockOut™ DMEM/F-12 basal medium, StemPro™ NSC SFM supplement, and recombinant growth factors (FGF basic and EGF) to provide optimal conditions for neural stem cell culture. The formulation includes the StemPro Neural Supplement, an optimized combination derived from N-2 and B-27 supplements to enhance neural stem cell proliferation and stability.
This medium supports both adherent culture systems and neurosphere suspension cultures, offering flexibility for various experimental workflows. Manufactured under cGMP standards, each lot is validated using a human neural stem cell proliferation assay, ensuring reliable and reproducible results for advanced stem cell research.
Key Features
- Complete serum-free medium kit for neural stem cell culture
- Optimized for human neural stem cell expansion and maintenance
- Supports multiple passages while preserving multipotency
- Enables differentiation into neurons, oligodendrocytes, and astrocytes
- Compatible with adherent and neurosphere suspension cultures
- Includes essential growth factors FGF-basic and EGF
- Manufactured under cGMP standards for consistency and reliability
- Lot-qualified using human neural stem cell proliferation assays
Applications
- Human neural stem cell expansion
- Neurosphere culture systems
- Neural differentiation studies
- Neurodevelopment research
- Stem cell biology and regenerative medicine research
- Drug discovery and neurotoxicity studies
- Disease modeling involving neural progenitor cells
Kit Components
| Component | Volume | Storage Conditions |
|---|---|---|
| KnockOut™ DMEM/F-12 Basal Medium | 500 mL | 2–8°C (store in the dark) |
| StemPro™ NSC SFM Supplement | 10 mL | −5 to −20°C |
| Recombinant Human FGF Basic | Included | 2–8°C (desiccated) |
| Recombinant Human EGF | Included | 2–8°C (desiccated) |
The kit provides sufficient reagents to prepare 500 mL of complete StemPro™ NSC culture medium.
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | StemPro™ NSC Serum-Free Medium |
| Brand | Gibco™ |
| Catalog Number | A1050901 |
| Cell Type | Human Neural Stem Cells (NSCs) |
| Culture Type | Mammalian cell culture |
| Medium Type | Serum-free |
| Culture Environment | CO₂ incubator |
| Endotoxin Level | ≤50 EU/mL |
| Form | Liquid |
| Species | Human |
| Quality Grade | Research grade |
| Additives | Low glucose, glutamine, phenol red, sodium pyruvate, sodium bicarbonate |
| Excludes | HEPES |
Advantages of StemPro™ NSC SFM
StemPro™ NSC SFM provides a highly optimized, defined culture environment that supports robust neural stem cell growth while maintaining the cells’ ability to differentiate into multiple neural lineages. The combination of optimized neural supplements and recombinant growth factors enables consistent expansion, improved reproducibility, and enhanced experimental reliability.
Gibco™ StemPro™ NSC Serum-Free Medium is a comprehensive solution for researchers working with human neural stem cells. With its optimized formulation, integrated growth factors, and cGMP manufacturing standards, it delivers reliable performance for neuroscience research, stem cell biology, and regenerative medicine applications. This complete medium system enables efficient NSC expansion while preserving their multipotent differentiation potential, making it an essential tool for advanced neural stem cell studies.