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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | antibody | Forms | Liquid |
| Product Brand | BD Biosciences | ||
| Product Grade | Molecular Biology | ||
BD™ CD4 PerCP (100 Tests)
The BD™ CD4 PerCP reagent is designed for in vitro diagnostic use in the identification of cells expressing the CD4 antigen, primarily in flow cytometry applications. This reagent leverages PerCP (Peridinin-Chlorophyll Protein), a fluorescent dye in the BD blue family, to provide high-performance fluorescence for precise cell analysis.
Key Features
- Intended Use:
- For in vitro diagnostics using BD FACS™ flow cytometers equipped with appropriate light scatter and fluorescence detection capabilities.
- Fluorescent Dye (PerCP):
- Excitation Max: 481 nm (excited by blue laser at 488 nm).
- Emission Max: 675 nm (detected using a 695/40 nm bandpass filter).
- Cross-laser excitation can occur with violet lasers (405 nm), resulting in spillover.
- Reagent Details:
- Isotype: Mouse BALB/c IgG1, κ.
- Concentration: 3 μg/mL.
- Volume Per Test: 20 μL.
- Applications:
- Designed for flow cytometry to identify human CD4-positive cells, including T-helper lymphocytes.
- Storage Conditions:
- Stable at 2° to 8°C.
- Do not freeze or expose to direct light.
Specifications
| Property | Details |
|---|---|
| Brand | BD™ |
| Alternative Names | L3T4, Leu3a, T4/Leu-3, W3/25 |
| Reactivity | Human |
| Excitation Source | Blue laser (488 nm) |
| Emission Max | 675 nm |
| Format | PerCP |
| Immunogen | Human Peripheral Blood T Cells |
| Concentration | 3 μg/mL |
| Storage Buffer | PBS with gelatin and 0.1% sodium azide |
| Regulatory Status | CE_IVD |
| Workshop Number | I T38; III T140,496 |
| Entrez Gene ID | 920 |
Dye Properties (PerCP)
| Feature | Details |
|---|---|
| Dye Family | BD blue family of dyes |
| Excitation Max | 481 nm |
| Emission Max | 675 nm |
| Optimal Laser Source | Blue laser (488 nm) |
| Detection Filter | 695/40 nm bandpass |
| Cross-Laser Excitation | Partial excitation by violet laser (405 nm) |
Applications
- Flow Cytometry:
- Detection and quantification of CD4+ T cells.
- Analysis of immune cell populations in human peripheral blood.
- Diagnostic Use:
- For in vitro diagnostic purposes, including monitoring immune system health.
Preparation and Handling
- Store at 2° to 8°C; avoid freezing.
- Protect from direct light to maintain reagent stability.
- Inspect for precipitation or discoloration, which indicates reagent deterioration.
- Ensure the flow cytometer is equipped with the appropriate laser and optical filter configurations for PerCP detection.
Important Notes
- Instrument Compatibility: Ensure the BD FACS™ cytometer is configured to detect the 481 nm excitation and 675 nm emission signals.
- Volume Per Test: Use 20 μL of the reagent for each test.
- Shelf Life: Do not use past the expiration date on the label.
The BD™ CD4 PerCP reagent combines the sensitivity of flow cytometry with the precision of PerCP fluorescence, making it an indispensable tool for immunological studies and diagnostics.