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Invitrogen™ Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP, 2mL
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Specifications:
| Application | Western Blot, ELISA-Based Assays, Immunohistochemistry, Immunoprecipitation (IP) | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Secondary Antibodies | Forms | Lyophilized |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP is an affinity-purified, HRP-conjugated polyclonal antibody optimized for Western Blot (WB), ELISA, IHC (P), and Immunoprecipitation (IP).
It reacts with the heavy chains of mouse IgG and the light chains common to most mouse immunoglobulins, ensuring broad secondary detection coverage while maintaining low cross-reactivity with non-Ig serum proteins.
This reagent has been validated in >2,700 publications and 84 figures, providing exceptional reliability and sensitivity for colorimetric or chemiluminescent HRP detection systems.
Key Features
- High Specificity: Recognizes mouse IgG heavy and light chains; minimal reactivity with non-Ig serum proteins.
- Robust HRP Conjugation: Delivers clear, high-intensity signal for colorimetric or ECL detection.
- Wide Assay Compatibility: Validated for WB, ELISA, IHC (P), and IP applications.
- Affinity-Purified: Reduces background for strong, reproducible results.
- Flexible Dilution Range: Broad dynamic detection (1:5,000–1:200,000 for WB).
- Lyophilized Format: Ensures long shelf life and shipping stability.
- Validated Performance: Tested lot-to-lot by ELISA and Immunoelectrophoresis (IEP).
Applications
| Application | Recommended Dilution Range |
|---|---|
| Western Blot (WB) | 1:5,000 – 1:200,000 |
| ELISA (Indirect/Sandwich) | 1:10,000 – 1:25,000 |
| Immunohistochemistry (Paraffin) | Assay-dependent |
| Immunoprecipitation (IP) | 1:500 – 1:5,000 |
Validated Example: Chemiluminescent Western Blot analysis of U-87 MG, SH-SY5Y, HeLa, and Hep G2 cell lysates shows specific detection of mouse IgG bands with minimal background signal.
Product Specifications
| Parameter | Specification |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Immunogen | Mouse IgG heavy and light chains |
| Class | Polyclonal |
| Conjugate | Horseradish Peroxidase (HRP) |
| Form | Lyophilized |
| Concentration (after reconstitution) | 0.8 mg/mL |
| Purification | Antigen Affinity Chromatography |
| Buffer Composition | PBS, pH 7.6 with 15 mg/mL BSA and 50 mM sucrose |
| Preservatives | None added |
| Reconstitution Volume | 2 mL distilled water |
| Storage Conditions | 4 °C (short term); –20 °C (long term in 50% glycerol) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| Cross-Reactivity | No reactivity with non-Ig serum proteins; possible minor cross-reactivity with Ig from other species |
Reconstitution & Storage
- Reconstitute lyophilized antibody in 2 mL of distilled water (0.8 mg/mL).
- Let stand 30 min at room temperature to fully dissolve.
- Mix gently and centrifuge to remove any particulates.
- Store at 4 °C for short-term use. For extended storage, add equal volume of glycerol (50% final) and store at –20 °C.
- Avoid repeated freeze-thaw cycles.
Target Information
Target: Mouse IgG (H+L)
Host Species: Goat
Conjugate: HRP (Horseradish Peroxidase)
Antibody Form: Whole Antibody (Polyclonal)
About Anti-Mouse Secondary Antibodies:
Anti-Mouse secondary antibodies enable specific detection of mouse primary antibodies across multiple platforms including Western blotting, immunofluorescence, ELISA, and immunohistochemistry. Their HRP conjugation facilitates signal amplification through chromogenic or chemiluminescent substrates, offering high sensitivity and reproducibility in protein and immunoassay applications.
Compliance and Use
- For Research Use Only. Not for use in diagnostic procedures or therapeutic applications.
The Invitrogen™ Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP (Cat. #31430) provides exceptional sensitivity and consistency for detecting mouse IgG primary antibodies in Western Blot, ELISA, IHC, and IP assays.
This affinity-purified, HRP-linked goat polyclonal delivers clean, high-intensity signals and is validated in thousands of peer-reviewed publications, making it a trusted choice for high-sensitivity protein detection in academic and biopharmaceutical research.