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Invitrogen™ UltraPure™ Tris Hydrochloride, 500g
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Specifications:
| Application | molecular biology | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Buffer | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Invitrogen™ UltraPure™ Tris Hydrochloride is a high-purity, molecular biology-grade reagent used for buffer preparation in biochemical and molecular biology applications. It is commonly used in nucleic acid gel electrophoresis, blotting, and other buffer-based experiments.
Tris Hydrochloride (tris(hydroxymethyl)aminomethane hydrochloride) provides excellent pH stability, making it ideal for preparing Tris-based buffers such as TAE (Tris-Acetate-EDTA), TBE (Tris-Borate-EDTA), and TE buffer (Tris-EDTA).
Key Features & Benefits
1. High-Purity UltraPure™ Tris Hydrochloride
- Molecular biology-grade purity ensures low contamination levels.
- Highly stable buffering capacity, suitable for long-term experiments.
- Ensures consistent pH control in biological reactions.
2. Versatile Buffering Agent for Molecular Biology
- Used in DNA and RNA electrophoresis buffers (TAE, TBE).
- Suitable for Western blotting and nucleic acid hybridization buffers.
- Helps maintain enzyme activity in molecular and biochemical assays.
3. Convenient & Reliable for Research Applications
- UltraPure™ quality guarantees batch-to-batch consistency.
- Easily dissolves in water, simplifying buffer preparation.
- Long shelf life when stored properly at room temperature.
Specifications
| Property | Details |
|---|---|
| Chemical Name | Tris Hydrochloride (tris(hydroxymethyl)aminomethane HCl) |
| Product Type | Tris Hydrochloride (HCl) Buffer |
| Form | Powder |
| Purity | Molecular Biology Grade |
| Applications | Nucleic Acid Gel Electrophoresis, Blotting |
| Product Line | UltraPure™ |
| Quantity | 500 g |
| Storage Conditions | Room Temperature |
| Shipping Condition | Room Temperature or Wet Ice |
| Unit Size | Each |
Applications
1. Nucleic Acid Electrophoresis & Blotting
- Key component in TAE and TBE buffers for DNA/RNA electrophoresis.
- Maintains pH stability during gel runs.
- Used in blotting techniques such as Southern and Northern blotting.
2. Biochemical & Molecular Biology Buffers
- Used in TE buffer (Tris-EDTA) for DNA/RNA storage.
- Maintains pH stability in enzyme assays and biochemical reactions.
- Helps preserve protein and nucleic acid integrity in lab workflows.
3. Enzyme Reactions & Protein Research
- Provides optimal buffering for restriction enzyme digestion.
- Stabilizes protein activity in molecular assays.
- Used in chromatography and protein purification techniques.
Pack Size & Catalog Number
| Pack Size | Catalog Number (SKU) |
|---|---|
| 500 g | 15506017 |
Storage & Handling
- Store at room temperature in a dry, well-sealed container.
- Dissolve in deionized water for buffer preparation.
- Adjust pH with HCl or NaOH as needed.
Summary
Invitrogen™ UltraPure™ Tris Hydrochloride is a high-quality buffer reagent used for molecular biology, nucleic acid electrophoresis, and biochemical applications. Its stable pH control and molecular biology-grade purity make it ideal for preparing Tris-based buffers such as TAE, TBE, and TE buffer.