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Thermo Scientific™ 5-Fluorouracil, 99%
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Antimetabolite Antineoplastic Agent and DNA Synthesis Inhibitor
Specifications:
| Application | cell Signalling, Apoptosis | ||
| Storage Temperature | -20°C | ||
| Product Type | Biochemical Reagent | Forms | Solid |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | Formula | C₄H₃FN₂O₂ |
Thermo Scientific™ 5-Fluorouracil (≥99%) is a synthetic fluorinated pyrimidine analogue of uracil used as an antimetabolite and antineoplastic research compound.
It functions by inhibiting the conversion of deoxyuridylic acid to thymidylic acid, blocking DNA synthesis and preventing cell proliferation. It can also be misincorporated into RNA, thereby impairing RNA processing and translation.
This reagent is ideal for use in tumor biology, DNA damage studies, immunosuppression models, and cytotoxic mechanism research.
Key Features
- High Purity: 98.5–101.0 % (NaOH titration, dried substance).
- Mechanism: Inhibits thymidylate synthase and blocks DNA synthesis.
- Dual Action: Interferes with both DNA and RNA metabolism.
- Physical Form: White to off-white crystalline powder.
- Low Impurities: Heavy metals ≤ 20 ppm; sulfated ash ≤ 0.1 %; loss on drying ≤ 0.5 %.
- Infrared Spectrum: Conforms to reference standard.
- Research Use Only: For laboratory or in-vitro applications; not for therapeutic use.
Chemical Identifiers
| Property | Value |
|---|---|
| IUPAC Name | 5-fluoro-1,2,3,4-tetrahydropyrimidine-2,4-dione |
| Synonyms | 5-FU, Fluorouracil, Uracil 5-fluoro- |
| CAS Number | 51-21-8 |
| Molecular Formula | C₄H₃FN₂O₂ |
| Molecular Weight | 130.08 g/mol |
| InChI Key | GHASVSINZRGABV-UHFFFAOYSA-N |
| SMILES | FC1=CNC(=O)NC1=O |
| MDL Number | MFCD00006018 |
Specifications
| Parameter | Specification |
|---|---|
| Purity | ≥ 98.5 % (on dried basis) |
| Form | Crystalline powder |
| Color | White to off-white |
| Heavy Metals (as Pb) | ≤ 20 ppm |
| Sulfated Ash | ≤ 0.1 % |
| Loss on Drying | ≤ 0.5 % (at 80 °C, 4 h vacuum) |
| Infrared Spectrum | Conforms |
| Solubility | Slightly soluble in water; soluble in polar solvents like DMSO |
| Storage | Store in a cool, dry place (2–8 °C) |
| Intended Use | Research Use Only (RUO) |
Applications
- Oncology Research: Study of tumor proliferation, DNA damage, and cytotoxicity.
- Immunology: Research on immunosuppressive effects and cell death pathways.
- Neuroscience: Investigations of 5-FU–induced neurotoxicity.
- Molecular Biology: Analysis of thymidylate synthase inhibition and RNA misincorporation.
- Pharmacology: Reference compound for antimetabolite mechanism studies.
Safety and Handling
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Classification (EC 1272/2008):
- Acute Toxicity (Oral) Cat. 3 | Skin Irritation Cat. 2 | Eye Irritation Cat. 2
- Germ Cell Mutagenicity Cat. 2 | Reproductive Toxicity Cat. 2
- Signal Word: Danger
- Hazard Statements: H301, H312 + H332, H315, H319, H335, H341, H361d
- Precautions: Wear protective gloves, eye protection, and lab coat; avoid ingestion and inhalation.
References
- Kato K. et al. “KEYNOTE-590: Phase III study of first-line chemotherapy with/without pembrolizumab for advanced esophageal cancer.” Future Oncol. 2019.
- Wagner A.D. et al. “Chemotherapy for advanced gastric cancer.” Cochrane Database Syst Rev. 2017, CD004064.
The Thermo Scientific™ 5-Fluorouracil, 99% is a research-grade antimetabolite used to study DNA synthesis inhibition, RNA incorporation, and tumor cytotoxicity.
With strict purity controls and low trace contaminants, it supports oncology, pharmacology, and molecular biology research requiring consistent and reliable performance.
- Pack Size: 1g 5g 25g