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Thermo Scientific™ Hippurate Disk, 25 Tests
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Specifications:
| Application | Antimicrobial Susceptibility and Resistance Testing (AST) |
| Storage Temperature | 2-8°C |
| Product Type | Antibiotic Disc |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Microbiology grade |
The Thermo Scientific™ Remel™ Hippurate Disk is a ready-to-use, reagent-impregnated diagnostic tool designed to detect the enzymatic hydrolysis of sodium hippurate by β-hemolytic streptococci (including Streptococcus agalactiae), Gardnerella vaginalis, and Campylobacter jejuni.
This rapid biochemical test provides results within 30 minutes, enabling fast and reliable differentiation of clinically significant organisms in diagnostic microbiology laboratories. The reaction is based on the detection of glycine released during hippurate hydrolysis, with a purple color indicating a positive result.
Key Features & Benefits
- Rapid Detection: Results available in as little as 30 minutes.
- Ready-to-Use Format: Disks are pre-impregnated with sodium hippurate and reagents for convenience.
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Clear Interpretation:
- Positive: Purple complex (hippurate hydrolysis present).
- Negative: Light gray color (no hydrolysis).
- Clinically Relevant: Differentiates Streptococcus agalactiae (Group B strep), Gardnerella vaginalis, and Campylobacter jejuni.
- CE Marked: Suitable for use in regulated clinical laboratories.
- Proven Methodology: Validated by peer-reviewed studies (Hwang & Ederer, 1975; Jolly, 1983; Cacho et al., 1989).
Principle of the Test
Hippuric acid is hydrolyzed by the enzyme hippuricase into glycine and benzoic acid.
- Glycine reacts with ninhydrin, triggering a series of reactions involving hydrindantin, ammonia, and residual ninhydrin.
- This condensation reaction produces a purple color for a positive result, confirming hippurate hydrolysis.
- Negative reactions remain light gray.
Applications
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Clinical Microbiology:
- Differentiation of Streptococcus agalactiae (Group B strep) in perinatal and neonatal screening.
- Identification of Campylobacter jejuni from enteric specimens.
- Biochemical confirmation of Gardnerella vaginalis.
- Diagnostic Laboratories: Rapid identification as part of routine bacterial biochemical testing panels.
- Educational & Reference Labs: Teaching tool for biochemical differentiation methods.
Technical Specifications
| Property | Details |
|---|---|
| Description | Hippurate Disk |
| Format | Vial |
| Packaging | 1 Kit |
| Quantity | 25 Tests/Kit |
| CE Marker | Yes |
| Unit Size | Each |
| Storage | 2°C to 8°C |
| Intended Use | Biochemical differentiation of Streptococcus agalactiae, G. vaginalis, and C. jejuni |
| Reaction Time | ~30 minutes |
| Result Interpretation | Purple = Positive; Gray = Negative |
References
- Hwang, M. & Ederer, G.M. (1975). J. Clin. Microbiol. 1:114–115.
- Jolly, J.L.S. (1983). J. Clin. Pathol. 36:476–478.
- Cacho, J.B. et al. (1989). J. Clin. Microbiol. 27:359–360.
- Greenwood, J.R. & Pickett, M.J. (1979). J. Clin. Microbiol. 9:200–204.
- MacFaddin, J.F. (2000). Biochemical Tests for Identification of Medical Bacteria. 3rd ed. Lippincott Williams & Wilkins.
The Thermo Scientific™ Remel™ Hippurate Disk offers a fast, reliable, and easy-to-interpret biochemical test for the differentiation of Group B Streptococcus, Gardnerella vaginalis, and Campylobacter jejuni. Its ready-to-use format, rapid turnaround time, and CE-marked compliance make it an indispensable tool for clinical microbiology laboratories performing routine bacterial identification.