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Sigma-Aldrich™ Murashige and Skoog (MS) Basal Medium (MS0), Powder
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Specifications:
| Application | Plant Cell Culture, Agricultural and horticultural research | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Plant Cell Culture | ||
The Sigma-Aldrich™ Murashige and Skoog (MS) Basal Medium (MS0) is a widely used plant tissue culture medium formulated according to the original Murashige and Skoog (1962) composition. This product contains the essential macro- and micronutrients and vitamins required for plant cell culture, providing a reliable foundation for a wide range of plant biotechnology applications.
Supplied as a basal formulation without sucrose or agar, this medium offers maximum flexibility, allowing researchers to customize culture conditions by adding carbon sources, gelling agents, and plant growth regulators as required.
Key Features
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Classic MS Formulation (MS0 Medium)
Includes essential nutrients and vitamins as per the original Murashige and Skoog formulation. -
Customizable Medium Composition
Designed for supplementation with sucrose, agar, auxins, and cytokinins. -
High Quality (Quality Level 200)
Ensures consistent and reproducible performance in plant tissue culture. -
Versatile Application Range
Suitable for a wide variety of plant species and experimental protocols. -
Powder Format for Easy Preparation
Enables precise control over medium composition and concentration.
Applications
- Plant tissue culture and micropropagation
- Plant transformation and genetic studies
- Callus induction and regeneration
- Shoot and root development studies
- Agricultural and plant biotechnology research
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Murashige and Skoog Basal Medium (MS0) |
| Synonyms | MS0 Medium, MSO Medium, MS Basal Medium |
| Brand | Sigma-Aldrich™ |
| SKU | M5519 |
| Form | Powder |
| Composition | MS macro- and micronutrients with vitamins |
| Preparation | 4.4 g per liter |
| Quality Level | 200 |
| Technique | Plant cell culture |
| Applications | Agriculture, plant biotechnology |
| Storage Temperature | 2–8 °C |
| MDL Number | MFCD00240976 |
Packaging & Ordering Information
| SKU | Pack Size | Description |
|---|---|---|
| M5519-1L | 1 L | Powder for preparation of 1 liter medium |
| M5519-10L | 10 L | Powder for preparation of 10 liters medium |
| M5519-50L | 50 L | Powder for preparation of 50 liters medium |
Preparation Guidelines (General)
- Dissolve 4.4 g of powder per liter of distilled water
- Add sucrose as a carbon source (commonly 20–30 g/L)
- Add agar or other gelling agents if required
-
Supplement with plant growth regulators such as:
- Auxins (e.g., IAA)
- Cytokinins (e.g., Kinetin)
- Adjust pH (typically 5.6 – 5.8)
- Sterilize by autoclaving
The Sigma-Aldrich™ Murashige and Skoog Basal Medium (MS0) provides a reliable and flexible foundation for plant tissue culture applications. Its customizable formulation allows researchers to tailor media composition to specific experimental needs, making it an essential medium for plant biotechnology, transformation studies, and micropropagation workflows.
- Pack Size: Powder for preparation of 10 liters medium Powder for preparation of 50 liters medium Powder for preparation of 1 liter medium