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Invitrogen™ Nitro blue Tetrazolium Chloride (NBT), 1g
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Specifications:
| Application | Western Blot, Colorimetric Assay | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Enzymes and Substrates | Forms | Solid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | Formula | C₄₀H₃₀Cl₂N₁₀O₆ |
Invitrogen™ Nitro Blue Tetrazolium Chloride (NBT) is a high-quality colorimetric chemical substrate used primarily to assess cell viability, metabolic activity, and redox potential. In redox-based assays, actively respiring cells reduce the water-soluble yellow NBT compound to an insoluble, deep blue formazan precipitate, providing a visible and quantifiable readout of cellular activity.
NBT is widely used in enzymatic staining protocols, including alkaline phosphatase (AP) and NADH/NADPH oxidase assays, as well as in Western blot detection and histochemistry applications for visualizing oxidative enzyme activity in tissues and cell cultures.
Key Features and Benefits
- Redox-Based Viability Assay: Ideal for measuring metabolic activity in live cells
- Visible Color Change: Produces a blue formazan precipitate upon reduction
- Versatile Use: Applicable in cell viability assays, enzyme detection, and tissue staining
- High Sensitivity: Detects low levels of oxidase or metabolic activity
- Ready for Standard Protocols: Compatible with alkaline phosphatase substrates such as BCIP
Applications
- Cell viability and respiration assays
- Redox potential evaluation
- Immunohistochemistry and cytochemistry
- Alkaline phosphatase (AP) detection in blots and tissues
- Detection of NAD(P)H-dependent oxidase activity
- Colorimetric staining in biological samples
Technical Specifications
| Property | Specification |
|---|---|
| Product Name | Nitro Blue Tetrazolium Chloride (NBT) |
| Catalog Number | N6495 |
| Quantity | 1 g |
| Detection Method | Colorimetric |
| Substrate Type | Oxidase substrate |
| Substrate Properties | Chemical substrate |
| Form | Solid |
| Storage Conditions | Store at room temperature, protect from light |
| Shipping Conditions | Ships at room temperature |
| Unit Size | Each |
Invitrogen™ Nitro Blue Tetrazolium Chloride (NBT) is a trusted substrate for colorimetric detection in a wide range of biochemical, cell-based, and histological assays. Whether used to monitor cell viability or detect oxidase enzyme activity, NBT delivers sensitive, reliable results with a straightforward, visual readout. A laboratory essential for redox research and diagnostic method development.