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Liofilchem® Ertapenem+Cloxacillin ET+CL, Antimicrobial Susceptibility Discs
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Specifications:
| Application | Clinical microbiology |
| Storage Temperature | -20°C |
| Product Type | Antibiotic Disc |
| Product Brand | Liofilchem |
| Product Grade | Microbiology grade |
Liofilchem® Ertapenem+Cloxacillin (ET+CL) Discs are specialized antimicrobial susceptibility discs designed to detect carbapenem resistance due to AmpC beta-lactamase production in Gram-negative organisms. The synergy between ertapenem (ET) and cloxacillin (CL) helps unmask the resistance mechanisms by inhibiting AmpC activity, making it an invaluable tool in clinical microbiology laboratories.
Key Features
- Dual Antibiotic Combination: Contains 10 µg ertapenem and cloxacillin to inhibit AmpC beta-lactamases.
- High Sensitivity: Specifically designed to detect AmpC-mediated resistance.
- Standardized Testing: Conforms to CLSI and EUCAST guidelines.
- User-Friendly: Suitable for routine diagnostic workflows using standard disc diffusion methods.
Technical Specifications
| Parameter | Details |
|---|---|
| Disc Content | Ertapenem 10 µg + Cloxacillin |
| Recommended Media | Mueller-Hinton Agar |
| Incubation Conditions | 35–37°C, 16–20 hours |
| Zone Diameter Reading | Measure in millimeters (mm) |
| Storage Conditions | -20°C for long-term storage; 2–8°C for short-term storage |
| Shelf-Life | Refer to packaging |
Test Procedure
- Preparation:
- Prepare a bacterial suspension equivalent to 0.5 McFarland standard in saline.
- Spread evenly onto a Mueller-Hinton Agar plate.
- Disc Placement:
- Place the ET+CL disc on the inoculated agar using sterile forceps or an automated dispenser.
- Ensure proper spacing between discs to prevent overlapping inhibition zones.
- Incubation:
- Incubate the plate at 35–37°C for 16–20 hours under aerobic conditions.
- Result Interpretation:
- Measure the diameter of the inhibition zone around the disc using a ruler or zone reader.
- Compare the zone diameters with those of ertapenem-only discs to observe synergy.
Result Interpretation
| Zone Diameter Difference | Interpretation |
|---|---|
| Significant increase (>5 mm) | Suggests AmpC-mediated carbapenem resistance |
| No increase or minimal change | Resistance mechanism likely not due to AmpC |
Quality Control
| Control Strains | Expected Zone Diameter (mm) |
|---|---|
| Escherichia coli ATCC® 25922 | 26–32 |
| Klebsiella pneumoniae ATCC® 700603 | 20–26 |
| Pseudomonas aeruginosa ATCC® 27853 | 17–23 |
Applications
- Detection of AmpC Beta-Lactamases:
- Differentiates between carbapenem resistance mechanisms.
- Screening for Carbapenemase-Producing Organisms (CPOs):
- Identifies resistance due to AmpC in Enterobacterales and Pseudomonas spp.
- Antimicrobial Resistance Monitoring:
- Contributes to global surveillance programs for multidrug-resistant (MDR) pathogens.
- Diagnostic Microbiology:
- Provides data for appropriate antimicrobial therapy selection.
Reference Data
| Study | Findings |
|---|---|
| CLSI M100 Performance Standards | Validated breakpoints for ertapenem testing |
| EUCAST Guidelines | Specific criteria for AmpC detection |
| Clinical Studies | High sensitivity and specificity for AmpC-related resistance |
Advantages
| Feature | Benefit |
|---|---|
| Combination Formulation | Enhances detection of AmpC-mediated resistance |
| Standardized Protocols | Ensures reliable and reproducible results |
| High Sensitivity | Detects low-level resistance mechanisms |
| Cost-Effective | Provides a phenotypic alternative to molecular testing |
Conclusion
The Liofilchem® Ertapenem+Cloxacillin ET+CL Discs are essential in detecting AmpC beta-lactamase-mediated resistance in clinical settings. Their compliance with international guidelines and robust performance make them a critical tool in combating antimicrobial resistance. By providing rapid, cost-effective phenotypic testing, they enhance laboratory workflows and support effective antimicrobial stewardship.