MCE β-Amylase, Bacillus subtilis, 1g

β-Amylase from Bacillus subtilis is a high-activity starch-degrading enzyme widely used in biochemical research, carbohydrate metabolism studies, industrial enzymology, and plant or microbial pathway investigations. This enzyme specifically catalyzes the exo-hydrolysis of α-1,4-glycosidic linkages in polysaccharides such as starch and glycogen, releasing maltose units from the non-reducing ends.

This microbial β-amylase displays robust activity under moderate acidic conditions (pH ~5.6) and elevated temperatures (≈60 °C), making it highly suitable for controlled starch processing reactions in experimental systems. As a degradative enzyme, it plays a key role in understanding saccharification, carbohydrate turnover, enzymatic hydrolysis kinetics, and fermentation feedstock optimization.

Biochemical Function

β-Amylase catalyzes:

• Exo-acting hydrolysis of α-1,4 glycosidic bonds
• Sequential release of maltose units
• Breakdown of gelatinized or soluble starch

Unlike α-amylase—an endo-acting enzyme—β-amylase produces defined maltose products, making it ideal for:

• Saccharide profiling
• Maltose production studies
• Enzymatic digestion assays
• Pathway mapping in carbohydrate metabolism

Origin

• Derived from microbial fermentation of Bacillus subtilis
• Produces consistent high activity and purity
• A preferred alternative to plant-derived β-amylases due to its stability and reproducible performance

Enzyme Activity

• ≥50 U/mg solid
• Unit Definition:
  One unit is defined as the amount of enzyme required to release 1 mmol of maltose from 2% soluble starch per hour at 60 °C, pH 5.6.

This standardized activity definition ensures precise reproducibility across experimental workflows.

Applications

1. Carbohydrate Metabolism & Biochemistry

• Study of starch degradation pathways
• Measurement of enzymatic kinetics
• Mapping of polysaccharide hydrolysis profiles

2. Industrial & Applied Research

• Maltose production systems
• Brewing and fermentation research
• Enzyme-assisted food processing
• Starch liquefaction and saccharification experiments

3. Analytical Research

• Determination of starch structure
• Monitoring starch breakdown in plant biology studies
• Quantifying enzymatic conversion efficiency

4. Comparative Enzymology

• Used in combination with α-amylase, glucoamylase, or cellulases to study synergistic polysaccharide degradation
• Useful for identifying substrate preference and enzyme kinetics

Specifications

MCE β-Amylase from Bacillus subtilis is a high-quality, high-activity microbial enzyme ideal for detailed starch degradation studies, enzymatic hydrolysis modeling, maltose production assays, and broad biochemical research applications. Its well-defined activity parameters, microbial origin, and excellent stability under assay conditions make it a reliable and versatile tool for laboratories working in enzyme kinetics, carbohydrate chemistry, plant sciences, fermentation research, and industrial biotechnology.