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Specifications:
| Application | neural stem cell culture | ||
| Storage Temperature | -20°C | ||
| Product Type | Supplement | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
The Gibco™ B-27™ Supplement, Minus Insulin (50X) is a customized serum-free neuronal culture supplement formulated from the original and most-cited B-27™ Supplement.
This variation excludes insulin, allowing researchers to culture hippocampal and central nervous system (CNS) neurons in experimental systems where insulin interferes with metabolic, signaling, or differentiation pathways.
Optimized for use with Gibco™ Neurobasal™ or Neurobasal™-A Media, this supplement supports consistent growth, morphology, and viability of neuronal cells under serum-free conditions while eliminating insulin-related pathway activation.
Key Features
- Insulin-free formulation: Ideal for neuronal cultures or assays sensitive to insulin signaling.
- Serum-free and defined: Ensures consistency and reproducibility across experiments.
- High-quality manufacturing: Produced under cGMP-compliant, ISO 13485-certified standards.
- Validated performance: Each lot tested for endotoxin, sterility, and mycoplasma.
- Compatible media: Designed for use with Neurobasal™ or Neurobasal™-A Medium.
- Flexible and reliable: Maintains neuron morphology and long-term viability.
- Shelf life: 12 months from date of manufacture.
- Storage: −5 to −20 °C; protect from light.
- Research Use Only (RUO): Not for diagnostic or therapeutic use.
Specifications
| Parameter | Specification |
|---|---|
| Cell Type | Neural cells (CNS, hippocampal) |
| Concentration | 50X |
| Culture Type | Mammalian cell culture |
| Product Line | B-27™ |
| Serum Level | Serum-free |
| Form | Liquid |
| Sterility | Sterile-filtered |
| Manufacturing Quality | cGMP-compliant, ISO 13485 certified |
| Tested For | Endotoxin, performance, sterility, mycoplasma |
| Quantity | 10 mL or 100 mL |
| Shelf Life | 12 months |
| Storage Conditions | −5 to −20 °C; protect from light |
| Shipping Conditions | Dry ice |
| Intended Use | Research Use Only (RUO) |
Applications
- Hippocampal and CNS neuronal culture under serum-free conditions
- Insulin-sensitive assays and metabolic studies
- Neuronal differentiation and survival assays without insulin interference
- Neurobiology and neurotoxicity testing
- Electrophysiology and synaptic function research
- Stem cell–derived neuronal culture systems requiring insulin-free conditions
The Gibco™ B-27™ Supplement, Minus Insulin (50X) delivers the trusted performance of the classic B-27™ formulation while enabling greater experimental flexibility in insulin-sensitive neuronal studies.
Produced under cGMP and ISO 13485 standards, it provides reproducible, serum-free conditions for cultivating hippocampal and CNS neurons without interference from insulin-mediated pathways—ideal for neurodevelopmental, metabolic, and electrophysiological research.
- Pack Size: 100 mL 10 mL