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Proteintech™ BCL2 Monoclonal Antibody (4H8C6), CoraLite® 594, 100 µL
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Specifications:
| Application | immunocytochemistry, Flow Cytometry | ||
| Storage Temperature | -20°C | ||
| Product Type | Primary Antibodies | Forms | Liquid |
| Product Brand | Proteintech | ||
| Product Grade | Molecular Biology | ||
The Proteintech BCL2 Monoclonal Antibody (Clone 4H8C6), CoraLite® 594 is a high-performance fluorescent antibody designed for the detection of BCL2 (B-cell lymphoma 2), a key regulator of apoptosis. Conjugated with CoraLite® 594, this antibody enables direct visualization of BCL2 expression in cells using fluorescence-based techniques such as immunocytochemistry (ICC/IF) and flow cytometry.
Targeting the full-length BCL2 protein, this monoclonal antibody provides high specificity and sensitivity for studying cell survival pathways, mitochondrial apoptosis regulation, and cancer progression mechanisms. Its red fluorescence emission (Ex/Em: 593/614 nm) makes it suitable for multiplex imaging applications alongside other fluorophores.
Key Features
- Specific for BCL2 apoptosis regulatory protein
- Monoclonal antibody clone 4H8C6
- Directly conjugated with CoraLite® 594 fluorophore
- Excitation/Emission: 593 / 614 nm (red fluorescence)
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Suitable for:
- Immunocytochemistry (ICC/IF): 1:50–1:500
- Flow cytometry (assay-dependent)
- Reactivity: Human, Pig
- High concentration: 2 mg/mL
- Eliminates need for secondary antibodies (reduced background, faster workflow)
- Ideal for multiplex fluorescence imaging
Applications
This antibody is recommended for:
- Immunofluorescence imaging of BCL2 in cultured cells
- Flow cytometry analysis of apoptosis-related proteins
- Studies of mitochondrial apoptosis pathways
- Investigation of BCL2 overexpression in cancer
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Research on:
- B-cell lymphoma
- Prostate cancer
- Cell survival and anti-apoptotic signaling
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Proteintech BCL2 Monoclonal Antibody (4H8C6), CoraLite® 594 |
| Catalog Number | CL594-60178 |
| Type | Antibody |
| Class | Monoclonal |
| Clone | 4H8C6 |
| Host / Isotype | Mouse / IgG2b |
| Species Reactivity | Human, Pig |
| Conjugate | CoraLite® 594 |
| Excitation / Emission | 593 / 614 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Amount | 200 µg |
| Volume | 100 µL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Preservative | 0.1% sodium azide |
| Storage Conditions | -20°C (protect from light) |
| Shipping Conditions | Wet ice |
Target Information
BCL2 is a central regulator of apoptosis that primarily functions as an anti-apoptotic protein, promoting cell survival by inhibiting mitochondrial pathways of cell death. It interacts with other BCL2 family members such as Bax and Bak, which promote apoptosis through cytochrome c release.
Overexpression of BCL2 is strongly associated with various cancers, particularly B-cell lymphomas, where chromosomal translocation leads to constitutive expression. BCL2 is also implicated in prostate cancer and other malignancies, making it a critical biomarker and therapeutic target in oncology research.
Availability Note
Important: This product is currently not available in certain regions. For procurement support or suitable alternatives, please contact LabMart or request equivalent products from other validated suppliers.
Advantages
- Direct fluorescent detection (no secondary antibody required)
- High specificity for apoptosis research
- Strong performance in ICC and flow cytometry
- Ideal for cancer and cell biology applications
- Compatible with multiplex fluorescence workflows
The Proteintech BCL2 Monoclonal Antibody (4H8C6), CoraLite® 594 is a powerful tool for researchers investigating apoptosis, cancer biology, and cell survival mechanisms. Its direct fluorophore conjugation, strong specificity, and optimized performance in fluorescence-based assays make it highly suitable for advanced imaging and flow cytometry applications.