Applied Biosystems™ TaqPath™ 1-Step RT-qPCR Master Mix, CG

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Specifications:

Application	Gene Expression, Multiplex qPCR
Storage Temperature	-15°C to -25°C
Product Type	PCR Master Mix	Forms	Liquid
Product Brand	Thermo Fisher Scientific™, Applied Biosystems™
Product Grade	Molecular Biology

Overview
Resource

Applied Biosystems™ TaqPath™ 1-Step RT-qPCR Master Mix, CG is a high-performance, single-tube 4X master mix designed for robust, sensitive, and reproducible one-step RT-qPCR workflows. It is suitable for pathogen detection, gene expression analysis, bioprocess quality control, adventitious agent testing, molecular diagnostics development, and other laboratory-based nucleic acid detection applications.

The formulation contains thermostable M-MLV reverse transcriptase, dNTPs, UNG, ROX passive reference dye, and thermostable Fast DNA Polymerase. This simplifies reaction setup by requiring only the addition of user-supplied assay and sample. The 4X concentration allows higher sample input per reaction, supporting sensitive detection of low-copy targets.

Key Features

Feature	Description
One-step RT-qPCR workflow	Reverse transcription and qPCR amplification occur in a single reaction
4X concentrated formulation	Allows increased sample input for improved sensitivity in low-volume reactions
High sensitivity	Detects targets down to 10 copies with reproducible CT results
Broad dynamic range	Supports up to 6 logs of dynamic range for RNA, DNA, and multiplexing applications
RNA and DNA target compatibility	Suitable for workflows involving RNA viruses, DNA viruses, cDNA, and DNA targets
Multiplex capability	Supports up to 3-plex reactions for multiple targets or control assays
Inhibitor tolerance	Performs reliably in the presence of common PCR inhibitors such as heparin, hematin, and EDTA
ROX included	ROX passive reference dye is pre-mixed for compatible qPCR platforms
UNG included	Supports carryover contamination control when used with dUTP-containing systems
Lot-to-lot consistency	Functionally tested to support reproducible CT performance and dynamic range

Product Variants

Catalog Number	Quantity	Approximate Reaction Capacity
A15299	5 × 1 mL	Sufficient for 1,000 × 20 µL reactions
A15300	1 × 10 mL	Sufficient for 2,000 × 20 µL reactions

Applications

Application Area	Typical Use
Pathogen detection	Detection of RNA and DNA microbial targets
Gene expression analysis	One-step RT-qPCR analysis of RNA transcripts
Molecular diagnostics development	Development and validation of laboratory molecular assays
Biopharmaceutical testing	Bioprocess QC, adventitious agent testing, and contamination monitoring
DNA quantification	Probe-based quantification of DNA targets
Presence/absence testing	Screening workflows for target detection
Multiplex qPCR	Detection of multiple targets or internal controls in a single reaction

Compatible Instruments

TaqPath™ 1-Step RT-qPCR Master Mix, CG is compatible with a range of Applied Biosystems™ real-time PCR systems, including:

Compatible Equipment
7500 Fast System
7500 System
7900HT System
QuantStudio™ 12K Flex
QuantStudio™ 3
QuantStudio™ 5
QuantStudio™ 6 Flex
QuantStudio™ 7
StepOne™
StepOnePlus™
ViiA™ 7 System
7500 Fast Dx System
QuantStudio™ Dx

Technical Specifications

Parameter	Specification
Product Name	Applied Biosystems™ TaqPath™ 1-Step RT-qPCR Master Mix, CG
Product Type	RT-qPCR Master Mix
Product Line	TaqPath™
PCR Method	1-step RT-qPCR
Detection Method	Primer-probe
Concentration	4X
Format	Tube
Form	Liquid
Reverse Transcriptase	M-MLV
Polymerase	Fast DNA Polymerase
Hot Start	Built-in Hot Start
Includes	dUTP, UNG/UDG
Passive Reference Dye	ROX, pre-mixed
Sample Type	RNA, cDNA, DNA
Target Specificity	RNA and DNA targets
Sensitivity	Down to 10 copies
Multiplex Capability	Up to 3-plex
Inhibitor Tolerance	High
GC-Rich PCR Performance	High
Reaction Speed	Fast or standard cycling
For Use With Applications	Microbial detection, DNA quantification, presence/absence testing
Storage	Store at -15°C to -25°C in the dark
Unit Size	Each
Use Statement	For Laboratory Use

Quality and Manufacturing

TaqPath™ 1-Step RT-qPCR Master Mix, CG is manufactured under a strong quality management system using traceable raw materials and validated operating procedures. It is produced in an ISO 13485-certified and FDA-registered facility that follows cGMP principles. All manufacturing lots are functionally tested to help ensure consistent CT performance, sensitivity, and dynamic range across assays.

Unique Advantages

Advantage	Benefit
Single protocol for RNA and DNA targets	Helps streamline workflows in laboratories testing both RNA and DNA pathogens
High-concentration 4X format	Supports higher sample input and improved low-copy target detection
Robust inhibitor tolerance	Increases confidence when working with complex clinical or biopharma samples
Fast or standard cycling compatibility	Provides workflow flexibility across different qPCR platforms
Integrated UNG/dUTP system	Helps reduce risk of carryover contamination
Compliance support	Compliance package availability supports internal quality and regulatory documentation needs

Applied Biosystems™ TaqPath™ 1-Step RT-qPCR Master Mix, CG is a robust, sensitive, and reproducible 4X master mix for one-step RT-qPCR applications. With built-in M-MLV reverse transcriptase, Fast DNA Polymerase, dUTP, UNG, and ROX dye, it simplifies assay setup while supporting high sensitivity, multiplexing, inhibitor tolerance, and broad compatibility with Applied Biosystems™ real-time PCR systems. It is well suited for microbial detection, gene expression, molecular assay development, biopharmaceutical QC, and laboratory testing workflows requiring consistent RT-qPCR performance.