Applied Biosystems™ SYBR™ Green PCR Master Mix

The Applied Biosystems™ SYBR™ Green Universal Master Mix is a high-performance, ready-to-use 2X qPCR master mix designed for reliable DNA amplification and detection using SYBR Green dye chemistry. It combines all essential components—including SYBR Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, ROX passive reference dye, and optimized buffer—into a single-tube format for streamlined workflow and consistent results.

Engineered for high sensitivity, specificity, and reproducibility, this master mix eliminates the need for probe-based assays, making it an efficient and cost-effective solution for gene expression studies, screening assays, and routine qPCR applications.

Key Features

• Complete 2X master mix for qPCR in a single tube
• Contains AmpliTaq Gold™ hot-start DNA polymerase for improved specificity
• SYBR Green I dye enables detection of double-stranded DNA without probes
• Includes ROX passive reference dye for signal normalization
• dUTP incorporation helps reduce carryover contamination
• Optimized buffer system for high sensitivity and reproducibility
• Compatible with a wide range of real-time PCR systems
• Supports GC-rich templates and flexible primer conditions

Applications

• Gene expression analysis (qPCR)
• DNA and cDNA quantification
• Screening assays and target validation
• Amplification of GC-rich sequences
• Routine molecular biology workflows

Product Variants

Technical Specifications

Compatibility

Compatible with a wide range of real-time PCR systems, including:

• Applied Biosystems™ 7000, 7300, 7500, 7700, 7900HT
• StepOne™ and StepOnePlus™ Systems
• QuantStudio™ Systems
• ViiA™ 7 System

Workflow Advantages

• Single-tube format reduces setup time and pipetting errors
• No need for target-specific probes, lowering assay costs
• Hot-start polymerase minimizes nonspecific amplification
• ROX normalization improves data consistency
• Supports flexible primer design (55–65°C Tm range)

The Applied Biosystems™ SYBR™ Green Universal Master Mix offers a robust and convenient solution for laboratories performing real-time PCR applications. Its optimized formulation ensures high sensitivity, specificity, and reproducibility, while the probe-free detection system provides flexibility and cost efficiency.

Ideal for both routine and advanced molecular workflows, this master mix enables laboratories to achieve reliable qPCR results with simplified setup and consistent performance.