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Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Proteins & Peptides | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Gibco™ Collagen I, Rat Tail is a high-quality, native extracellular matrix protein used to promote cell adhesion, growth, differentiation, and migration in cell culture. As the most abundant fibrillar collagen—making up approximately 90% of collagen in skin, bone, tendon, and other connective tissues—collagen I provides an optimal microenvironment for primary and stem cell culture.
Sourced from rat tail tendon, this collagen is supplied in liquid form at 3 mg/mL, allowing flexibility for direct use or dilution to desired working concentrations. It can be gelled into a firm, transparent 3D matrix or used for coating tissue culture surfaces to support a variety of cell types, including keratinocytes, hepatocytes, fibroblasts, and epithelial cells.
Key Features
- High-Purity Native Collagen – Maintains structural integrity for reliable cell culture performance.
- Flexible Format – Supplied at 3 mg/mL for easy dilution or direct use.
- 3D & 2D Applications – Forms firm, clear gels or surface coatings for cell attachment.
- Optimal for ECM Studies – Supports cell proliferation, migration, and differentiation.
- Endotoxin Controlled – ≤10 EU/mL for reduced interference in sensitive assays.
Applications
- 3D Cell Culture – Create collagen gels for tissue-like growth environments.
- 2D Surface Coating – Enhance adhesion of anchorage-dependent cells.
- Stem Cell Research – Maintain and differentiate pluripotent or multipotent cells.
- Primary Cell Culture – Support hepatocytes, keratinocytes, and fibroblasts.
- Wound Healing & Migration Assays – Study ECM-driven cell behaviors.
- Tissue Engineering Models – Build ECM-mimicking culture systems.
Technical Specifications
| Parameter | Specification |
|---|---|
| Protein Type | Collagen I |
| Source | Rat tail tendon |
| Concentration | 3–4 mg/mL |
| Form | Liquid |
| Protein Form | Native |
| Endotoxin Level | ≤10 EU/mL |
| Species | Rat |
| Applications | Cell culture, ECM studies |
| Storage | 2–8°C, protect from light |
| Shipping | Wet ice |
| Quantity | 20–25 mL |
The Gibco™ Collagen I, Rat Tail is a trusted ECM substrate for both 3D and 2D cell culture applications, providing a biologically relevant matrix to support cell attachment, proliferation, and differentiation. Its native structure and controlled purity make it ideal for research in cell biology, regenerative medicine, and tissue engineering.