Liofilchem® Chromatic™ CRE Supplement, 10 x 10 mL

Liofilchem® Chromatic™ CRE Supplement is a selective lyophilized additive designed for the preparation of Chromatic™ CRE Agar Base (Ref. 611619 / 621619). The complete medium enables the isolation and differentiation of carbapenem-resistant Enterobacteriaceae (CRE) directly from clinical specimens, aiding in the early detection of multidrug-resistant pathogens such as Klebsiella pneumoniae (KPC), Escherichia coli (NDM, OXA, VIM), and other carbapenemase-producing Enterobacteriaceae.

The selective formulation enhances the recovery of CRE while inhibiting carbapenem-susceptible and non-Enterobacteriaceae species, ensuring high diagnostic accuracy and reproducibility in antimicrobial resistance surveillance and infection control applications.

Key Features

• Highly selective supplement for CRE detection and differentiation.
• For use with Chromatic™ CRE Agar Base (Ref. 611619 / 621619).
• Facilitates direct detection of carbapenem-resistant Enterobacteriaceae from clinical specimens.
• Suppresses non-Enterobacteriaceae and carbapenem-sensitive strains.
• Lyophilized format—ready to reconstitute and incorporate into base medium.
• ISO and EUCAST guideline compliant.
• Each vial sufficient for 500 mL of medium.
• Ideal for clinical microbiology and hospital infection control laboratories.

Composition

Principle of the Method

Carbapenem-resistant Enterobacteriaceae are among the most critical multidrug-resistant pathogens in clinical settings. The Chromatic™ CRE Supplement contains a specialized selective mix that enhances the recovery of carbapenemase-producing Enterobacteriaceae (KPC, NDM, VIM, OXA-48 types), while suppressing carbapenem-susceptible strains and unrelated flora.

When incorporated into Chromatic™ CRE Agar Base, the supplement allows for the visual differentiation of CRE colonies, typically forming blue-violet colonies on the chromogenic base medium, providing a rapid and reliable screening method consistent with EUCAST and CLSI recommendations.

Preparation and Use

1. Reconstitute one vial of Chromatic™ CRE Supplement aseptically with 5 mL of sterile distilled water.
2. Mix until completely dissolved.
3. Add the entire reconstituted vial aseptically to 500 mL of autoclaved Chromatic™ CRE Agar Base (45–50 °C).
4. Mix gently and pour into sterile Petri dishes.

Quality Control

Appearance: Lyophilized, whitish button; clear solution after reconstitution.

Incubation: 18–24 hours at 35 ± 2 °C (aerobic).

Storage and Stability

• Store at 2–8 °C in original sealed packaging.
• Protect from humidity and light.
• Stable during transport for up to 4 days at 18–25 °C or 48 hours at 35–39 °C.
• Shelf life: As indicated on product label (typically 4 years).
• Discard if signs of contamination or deterioration occur.

Safety and Handling

• For in vitro diagnostic use only.
• Use by trained microbiology professionals in accordance with biosafety protocols.
• Consult the Safety Data Sheet (SDS) before handling.
• Dispose of media and biological waste per local biosafety and environmental regulations.

Ordering Information

Liofilchem® Chromatic™ CRE Supplement, when combined with Chromatic™ CRE Agar Base, provides a highly specific, rapid, and efficient solution for the detection of carbapenem-resistant Enterobacteriaceae (CRE). The formulation ensures selective isolation of clinically significant carbapenemase-producing strains, supporting infection prevention, epidemiological monitoring, and antimicrobial stewardship programs in healthcare and public health laboratories.