Liofilchem® Campylobacter Growth Supplement, 10 x 10 mL

Liofilchem® Campylobacter Growth Supplement is a freeze-dried additive designed to enhance the growth and recovery of Campylobacter spp., including C. jejuni and C. coli, on culture media such as Campylobacter Agar Base (ref. 611007 / 621007).

The supplement contains sodium pyruvate, sodium metabisulphite, and ferrous sulphate, which neutralize toxic oxygen derivatives (superoxide anions, peroxides) that inhibit Campylobacter growth in standard culture conditions. By improving aerotolerance and detoxifying reactive oxygen species, this supplement ensures rapid and efficient isolation of Campylobacter species from clinical, food, and environmental samples.

Key Features

• Enhances growth and recovery of Campylobacter spp. under microaerophilic conditions.
• Neutralizes toxic oxygen species, improving aerotolerance.
• Compatible with Campylobacter Agar Base, Columbia Agar, or Blood Agar with selective supplements.
• Freeze-dried and easy to reconstitute, ensuring batch-to-batch consistency.
• Each vial sufficient for 500 mL of medium.
• Validated for diagnostic and food microbiology laboratories.

Composition

Principle of the Method

Campylobacter species are microaerophilic bacteria requiring reduced oxygen tension (~6%) for optimal growth.

Toxic oxygen radicals often inhibit their development in standard aerobic conditions.

The addition of ferrous sulphate, sodium metabisulphite, and sodium pyruvate binds and inactivates these reactive species, allowing Campylobacter jejuni/coli to grow efficiently on selective or enriched media.

This growth supplement enhances recovery, particularly when combined with selective antibiotics and blood enrichment, for rapid detection and identification.

Preparation and Use

1. Aseptically reconstitute one vial with 5 mL of sterile distilled water.
2. Shake gently until completely dissolved (avoid foaming).
3. Add the entire 5 mL to 500 mL of autoclaved Campylobacter Agar Base (45–50 °C).
4. If required, add 5–10% lysed horse or ram blood and the appropriate selective supplement for Campylobacter isolation.
5. Mix carefully and pour into Petri dishes under aseptic conditions.

Quality Control

Appearance: greenish freeze-dried powder

Incubation conditions: 48 h at 42 °C, microaerophilic atmosphere

Storage and Stability

• Store at 2–8 °C in original packaging.
• Protect from moisture and direct light.
• Shelf life: Valid until expiry date on the label (typically 4 years).
• Do not use if deterioration or contamination is evident.

Safety and Handling

• Contains chemical reducing agents; handle with care.
• For in vitro diagnostic use only.
• Use by trained personnel under aseptic laboratory conditions.
• Refer to Safety Data Sheet (SDS) for detailed handling and disposal instructions.

Ordering Information

Liofilchem® Campylobacter Growth Supplement provides an optimized, reliable formulation that enhances the isolation and recovery of Campylobacter spp. by neutralizing oxygen toxicity in culture environments. When combined with Campylobacter Agar Base and selective additives, it delivers consistent and reproducible growth results, making it essential for clinical, food safety, and veterinary diagnostic laboratories involved in Campylobacter testing.