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VWR Ethidium bromide stock solution, Biotechnology Grade
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CAS Number :
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Formula: C₂₁H₂₀BrN₃
CAS Number: 1239-45-8
Specifications:
| Application | molecular biology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Gel Stain | Forms | Liquid |
| Product Brand | VWR | ||
| Product Grade | Molecular Biology | Formula | C₂₁H₂₀BrN₃ |
Fluorescent DNA/RNA Stain for Gel Electrophoresis & Nucleic Acid Visualization
VWR Ethidium Bromide (EtBr) Stock Solution is a 10 mg/mL solution of high-purity EtBr in deionized water, used as a fluorescent intercalating dye for nucleic acid staining. It is the gold standard for detecting DNA and RNA in agarose or acrylamide gels under UV illumination. This solution is ready-to-use and ideal for molecular biology protocols requiring high sensitivity and reliable band visualization.
Key Features
- Biotechnology Grade Purity (≥98%)
- Pre-diluted 10 mg/mL stock solution – Ready for immediate use
- Highly Sensitive – Detects as little as 10 ng of DNA
- No RNase or DNase contamination – Ideal for molecular applications
- Long shelf life – Stable at ambient temperature
Specifications
| Property | Value |
|---|---|
| Concentration | 10 mg/mL in deionized water |
| Purity (Dry Basis) | ≥98% |
| DNase/RNase Activity | None detected |
| Moisture Content | ≤8% |
| Detection Sensitivity | ≥10 ng nucleic acid |
| Storage Temperature | Ambient |
| Molecular Formula | C₂₁H₂₀BrN₃ |
| Molar Mass | 394.31 g/mol |
| CAS Number | 1239-45-8 |
| Grade | Biotechnology Grade |
Applications
- DNA and RNA Visualization in agarose/acrylamide gels
- Gel Electrophoresis – Standard stain for nucleic acid bands
- Nucleic Acid Quantification and Detection
- DNA Purification Support in CsCl gradients
- Molecular Biology & Genomic Research
Usage Guidelines
- Use at 0.05 μg/mL for gel staining
- Use at 0.5–1.0 μg/mL for nucleic acid purification
- Observe under UV light (~302 nm) for best visualization
- Follow laboratory safety protocols when handling EtBr
VWR® Ethidium Bromide Stock Solution offers consistent quality, ease of use, and high sensitivity for DNA/RNA detection in electrophoresis. Its biotech-grade formulation and nuclease-free preparation make it a trusted reagent for molecular biology applications.