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Pierce Protein A Plus Agarose delivers enhanced performance in terms of binding capacity and protein recovery for researchers performing small to medium-scale antibody purification. It is especially suited for purifying polyclonal IgG from human, rabbit, dog, cat, and pig serum.
Each 1 mL of resin can bind ≥34 mg of human IgG, enabling robust purification workflows in standard microcentrifuge or chromatography columns.
Key Features & Benefits
-
High Capacity Binding
≥34 mg human IgG/mL resin; 16–17 mg mouse IgG/mL resin. -
Wide Species Compatibility
Ideal for rabbit, pig, dog, cat, and human IgG subclasses (except human IgG3 and mouse IgG1, which bind weakly). -
Superior Resin Support
Crosslinked 6% beaded agarose offers a strong, stable matrix for affinity chromatography. -
Low Nonspecific Binding
Leach-resistant, charge-free covalent Protein A immobilization reduces background and improves purity. -
Reusable Resin
Stable across pH 2–14 (short term) or 3–13 (long term); can be used in multiple cycles with consistent results.
Protein A Characteristics
- Source: Derived from Staphylococcus aureus.
- Structure: 46.7 kDa single-chain polypeptide with four high-affinity Fc-binding domains.
- Affinity: Binds selectively to the Fc region of IgG antibodies.
Applications
- Rapid small-scale purification of polyclonal or monoclonal IgG
- Antibody recovery from serum, ascites fluid, or hybridoma culture supernatants
- Immunoprecipitation workflows
- Protein complex isolation studies
Specifications
| Property | Detail |
|---|---|
| Format | Bottle (ready-to-use slurry) |
| Column Type | Agarose Resin (Affinity) |
| Stationary Phase | Protein A on 6% crosslinked agarose |
| Volume Options | 1 mL, 5 mL, 25 mL |
| Average Particle Size | 45–165 µm |
| Binding Capacity | ≥34 mg human IgG/mL |
| pH Stability | 2–14 (short), 3–13 (long term) |
| Operating Pressure | ≤25 psi |
| Storage Temp | 2°C to 8°C |
Pierce™ Protein A Plus Agarose is a powerful and reliable affinity resin that delivers high-yield IgG purification with minimal sample loss or contamination. Its high capacity, broad species compatibility, and robust chemical stability make it a top choice for academic and industrial labs performing immunoglobulin purification from a wide variety of sources. For rapid workflows, the resin is also available pre-packed in NAb™ Spin Columns with compatible buffers.
User Guide: Pierce Protein A Agarose
Pierce Protein A Plus Agarose delivers enhanced performance in terms of binding capacity and protein recovery for researchers performing small to medium-scale antibody purification. It is especially suited for purifying polyclonal IgG from human, rabbit, dog, cat, and pig serum.
Each 1 mL of resin can bind ≥34 mg of human IgG, enabling robust purification workflows in standard microcentrifuge or chromatography columns.
Key Features & Benefits
-
High Capacity Binding
≥34 mg human IgG/mL resin; 16–17 mg mouse IgG/mL resin. -
Wide Species Compatibility
Ideal for rabbit, pig, dog, cat, and human IgG subclasses (except human IgG3 and mouse IgG1, which bind weakly). -
Superior Resin Support
Crosslinked 6% beaded agarose offers a strong, stable matrix for affinity chromatography. -
Low Nonspecific Binding
Leach-resistant, charge-free covalent Protein A immobilization reduces background and improves purity. -
Reusable Resin
Stable across pH 2–14 (short term) or 3–13 (long term); can be used in multiple cycles with consistent results.
Protein A Characteristics
- Source: Derived from Staphylococcus aureus.
- Structure: 46.7 kDa single-chain polypeptide with four high-affinity Fc-binding domains.
- Affinity: Binds selectively to the Fc region of IgG antibodies.
Applications
- Rapid small-scale purification of polyclonal or monoclonal IgG
- Antibody recovery from serum, ascites fluid, or hybridoma culture supernatants
- Immunoprecipitation workflows
- Protein complex isolation studies
Specifications
| Property | Detail |
|---|---|
| Format | Bottle (ready-to-use slurry) |
| Column Type | Agarose Resin (Affinity) |
| Stationary Phase | Protein A on 6% crosslinked agarose |
| Volume Options | 1 mL, 5 mL, 25 mL |
| Average Particle Size | 45–165 µm |
| Binding Capacity | ≥34 mg human IgG/mL |
| pH Stability | 2–14 (short), 3–13 (long term) |
| Operating Pressure | ≤25 psi |
| Storage Temp | 2°C to 8°C |
Pierce™ Protein A Plus Agarose is a powerful and reliable affinity resin that delivers high-yield IgG purification with minimal sample loss or contamination. Its high capacity, broad species compatibility, and robust chemical stability make it a top choice for academic and industrial labs performing immunoglobulin purification from a wide variety of sources. For rapid workflows, the resin is also available pre-packed in NAb™ Spin Columns with compatible buffers.
User Guide: Pierce Protein A Agarose
![[21012] Thermo Scientific™ Pierce™ Gentle Ag/Ab Binding Buffer, pH 8.0 (3.75L)](/web/image/product.product/43552/image_512/%5B21012%5D%20Thermo%20Scientific%E2%84%A2%20Pierce%E2%84%A2%20Gentle%20Ag-Ab%20Binding%20Buffer%2C%20pH%208.0%20%283.75L%29?unique=96c5da2)
![[21013] Thermo Scientific™ Pierce™ Gentle Ag/Ab Elution Buffer (3.75L)](/web/image/product.product/43550/image_512/%5B21013%5D%20Thermo%20Scientific%E2%84%A2%20Pierce%E2%84%A2%20Gentle%20Ag-Ab%20Elution%20Buffer%20%283.75L%29?unique=96c5da2)
![[21030] Thermo Scientific™ Pierce™ Gentle Ag/Ab Binding and Elution Buffer Kit (100 mL Each)](/web/image/product.product/43553/image_512/%5B21030%5D%20Thermo%20Scientific%E2%84%A2%20Pierce%E2%84%A2%20Gentle%20Ag-Ab%20Binding%20and%20Elution%20Buffer%20Kit%20%28100%20mL%20Each%29?unique=96c5da2)
![[44667] Thermo Scientific™ Pierce™ Protein A IgG Purification Kit](/web/image/product.product/43531/image_512/%5B44667%5D%20Thermo%20Scientific%E2%84%A2%20Pierce%E2%84%A2%20Protein%20A%20IgG%20Purification%20Kit?unique=96c5da2)
![[89952] Thermo Scientific™ NAb™ Protein Spin Columns, 0.2 mL (Protein A Plus, ≥7.0 mg IgG)](/web/image/product.product/43533/image_512/%5B89952%5D%20Thermo%20Scientific%E2%84%A2%20NAb%E2%84%A2%20Protein%20Spin%20Columns%2C%200.2%20mL%20%28Protein%20A%20Plus%2C%20%E2%89%A57.0%20mg%20IgG%29?unique=96c5da2)
规格
| Pack Size | 1 mL, 5 mL, 12 mL |