Thermo Scientific™ Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

For sensitive, high-specificity RNA target amplification in a single-tube TaqMan™ real-time RT-PCR format

The AgPath-ID™ One-Step RT-PCR Reagents provide a fast, reliable, and sensitive solution for the detection and quantitation of RNA targets. Designed for research applications involving RNA pathogen amplification, this kit combines ArrayScript™ Reverse Transcriptase—a mutant MMLV RT engineered for high cDNA yields—with AmpliTaq Gold™ DNA Polymerase, the gold-standard hot-start enzyme for specific target amplification.

Optimized to work with user-supplied primers and probes, the kit supports rapid setup and results in approximately one hour, minimizing handling errors and ensuring consistent, reproducible outcomes. The included ROX™ passive reference dye allows for quantitative fluorescent signal normalization across compatible real-time PCR platforms.

Key Features & Benefits

• High Sensitivity & Specificity – Detects low-copy RNA targets with robust and reproducible amplification.
• Single-Tube Reaction Format – Reduces sample handling steps, minimizing contamination risk.
• Fast Results – Complete reverse transcription and PCR in ~1 hour.
• Optimized Performance – Works with a broad range of Applied Biosystems™ real-time PCR instruments; optimized for the 7500 System.
• Integrated ROX™ Reference Dye – Enables accurate fluorescent signal normalization in quantitative assays.
• High-Yield Reverse Transcription – Powered by ArrayScript™ Reverse Transcriptase for efficient cDNA synthesis.
• Reliable Amplification – Utilizes AmpliTaq Gold™ DNA Polymerase for hot-start, high-specificity PCR.

Applications

• Detection and quantification of RNA pathogens
• Gene expression analysis from RNA templates
• Molecular diagnostics research
• High-throughput real-time PCR assays

Technical Specifications

Ordering Information

The Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents deliver unmatched convenience, speed, and reliability for RNA target detection in real-time PCR workflows. By combining a high-efficiency reverse transcriptase with the proven specificity of AmpliTaq Gold™ DNA Polymerase in a single-tube format, these reagents minimize workflow complexity while maximizing sensitivity. Whether used for pathogen surveillance, gene expression studies, or other RNA-based research, AgPath-ID™ offers a robust and reproducible solution trusted by molecular biology laboratories worldwide.