Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR

The TaqMan™ Fast Virus 1-Step Master Mix is a powerful reagent designed for fast and highly sensitive real-time reverse transcription polymerase chain reaction (RT-PCR) applications. It is optimized for the detection and quantification of RNA and DNA viruses, even in the presence of challenging PCR inhibitors. Its versatile and efficient one-step format is ideal for high-throughput workflows and multiplexed assays.

Key Features:

1. High Sensitivity:
   • Optimized 4X master mix formulation enhances detection of both RNA and DNA viruses.
   • Suitable for low-titer viral nucleic acids with the ability to handle smaller reactions or larger sample inputs.
2. Consistency with Inhibitors:
   • Formulated to overcome inhibitors commonly found in research samples such as blood, stool, and tissue.
   • Ensures reliable performance even with difficult sample types.
3. Simplified Protocol:
   • One-tube, one-step reaction format for streamlined handling.
   • Compatible with both RNA and DNA virus detection in a single plate, reducing workflow complexity.
4. Flexibility:
   • Effective for singleplex and multiplex assays, supporting up to three probes (triplex reactions) or higher-order multiplexing with the appropriate master mix formulation.
   • Compatible with exogenous or endogenous internal controls.
5. Fast Results:
   • High-concentration master mix allows for smaller reaction volumes, enabling fast cycling protocols without sacrificing sensitivity or accuracy.
   • Achieve qPCR cycling in under 30 minutes for increased throughput.
6. Two Formulations:
   • TaqMan Fast Virus 1-Step Master Mix with ROX dye: For singleplex and triplex reactions using passive reference dyes.
   • TaqMan Fast Virus 1-Step Multiplex Master Mix (No ROX): For higher-order multiplexing or JUN dye-based assays.
7. Robust and Reliable:
   • Built-in hot start with AmpliTaq™ Fast DNA Polymerase ensures precision and efficiency.
   • Suitable for challenging environments with high inhibitor tolerance.

Applications:

• Gene expression studies.
• Virus detection and quantification.
• High-throughput qPCR workflows.
• Multiplex assays for complex sample analysis.

Specifications:

Packaging Options:

Contents:

• AmpliTaq™ Fast DNA Polymerase, UP
• Thermostable M-MLV Reverse Transcriptase
• dNTPs
• RNase Inhibitor
• ROX™ dye (passive reference)

Storage and Stability:

• Store at -15°C to -25°C in the dark for optimal stability.

The TaqMan™ Fast Virus 1-Step Master Mix delivers high performance in virus detection and gene expression studies, providing reliable results even under challenging conditions. Its flexibility, speed, and sensitivity make it a trusted solution for researchers tackling complex viral analyses.