Invitrogen™ Biotin-HRP (biotinylated peroxidase), 5mg

Invitrogen™ Biotin-HRP is a high-quality biotinylated horseradish peroxidase (HRP) conjugate designed for sensitive and specific detection of streptavidin and avidin conjugates in a wide range of biological assays. It combines the strong affinity of biotin for avidin/streptavidin with the signal amplification capability of HRP, making it an essential reagent for biotin-based detection systems.

Biotin-HRP binds specifically to streptavidin or avidin-labeled targets, enabling its use in various colorimetric, chemiluminescent, and fluorescent enzyme-based detection systems.

Key Features & Benefits

• High Affinity & Specificity
  Reacts efficiently with streptavidin and avidin conjugates, enabling sensitive biotin-streptavidin based assays.
• Powerful Enzymatic Amplification
  HRP provides a robust catalytic activity with commonly used substrates like TMB, DAB, and chemiluminescent reagents, delivering strong signal intensity.
• Ready-to-Use Liquid Form
  Supplied as a stable liquid formulation for convenient and reproducible application.
• Versatile Applications
  Suitable for a wide range of immunoassays including ELISA, Western blotting, immunohistochemistry (IHC), and nucleic acid detection.
• Reliable Storage & Handling
  Maintains activity when stored at 2–8°C; no reconstitution required.

Applications

• ELISA (Enzyme-Linked Immunosorbent Assays)
  Detect biotinylated capture antibodies or antigens through streptavidin or avidin conjugates.
• Western Blotting
  Visualize biotinylated secondary antibodies or probes using HRP-based chemiluminescence.
• Immunohistochemistry (IHC)
  Detect biotinylated primary antibodies in tissue sections with HRP and DAB substrate.
• Dot Blots & Slot Blots
  Sensitive detection of nucleic acid or protein targets labeled with biotin.
• Nucleic Acid Hybridization
  Signal amplification via biotinylated probes for Southern or Northern blots.

Storage & Stability

• Storage: Store between 2°C and 8°C in the original container to maintain activity.
• Stability: Stable under recommended conditions; avoid freeze-thaw cycles.
• Shipping: Ships on wet ice for temperature control during transit.

Invitrogen™ Biotin-HRP offers a reliable and high-performance solution for researchers seeking a sensitive biotin detection reagent. With a well-characterized reactivity profile, stable liquid formulation, and robust enzyme activity, it supports a broad array of biochemical and immunological applications. Whether used in ELISA, blotting, or IHC, this reagent ensures consistent and reproducible results.