Thermo Scientific™ Pierce™ Biotin (Unconjugated), 1g

Thermo Scientific™ Pierce™ Biotin is a high-purity, unconjugated form of biotin (vitamin H), a small, essential cofactor present in all living cells. Its compact size and versatile carboxylic acid functional group make it ideal for chemical derivatization and covalent conjugation to proteins, nucleic acids, enzymes, and other biomolecules.

This biotin product serves as a foundational reagent in a variety of biotin-streptavidin-based applications, including antibody labeling, enzyme conjugation, affinity purification, and detection workflows.

Key Features & Benefits

• Highly Reactive Functional Group
  Features a carboxylic acid moiety suitable for coupling to amines, hydrazides, or other reactive groups via carbodiimide or NHS-ester chemistry.
• Versatile Solubility
  Soluble in DMSO, DMF, and water, facilitating its use in both aqueous and organic reaction systems.
• Flexible for Conjugation Chemistry
  The valeric acid side chain allows modification with reactive handles (e.g., NHS, maleimide, azide), enabling downstream labeling or immobilization.
• Essential for Biotin-Avidin Assays
  Forms strong, non-covalent bonds with avidin and streptavidin proteins—widely used in immunoassays, Western blots, ELISAs, and microscopy.
• Unconjugated Format for Custom Use
  Enables researchers to design and execute custom biotinylation strategies tailored to specific targets and experimental systems.

Applications

• Antibody Biotinylation
  Covalently label antibodies for detection or immobilization in streptavidin-based systems.
• Protein Labeling & Detection
  Prepare custom biotin-protein conjugates for use in pull-down assays, protein interaction studies, or surface capture experiments.
• Nucleic Acid Probes
  Incorporate biotin into DNA or RNA oligonucleotides for hybridization assays and molecular probe development.
• Affinity Purification
  Use biotinylated molecules to selectively capture and isolate streptavidin-coated beads or columns.
• Immunohistochemistry (IHC)
  Biotin can be conjugated to enzyme reporters (e.g., HRP or AP) or antibodies for signal amplification.

Specifications

Thermo Scientific™ Pierce™ Biotin provides a reliable and flexible solution for custom labeling and detection strategies using the biotin-avidin/streptavidin system. Its high chemical reactivity, broad solubility, and compatibility with various conjugation chemistries make it indispensable in protein engineering, immunoassays, and biochemical research workflows. Ideal for researchers needing a foundational biotin reagent for sensitive and specific molecular interactions.