Thermo Scientific™ Pierce™ Gentle Ag/Ab Binding and Elution Buffer Kit (100 mL Each)

Thermo Scientific™ Pierce™ Gentle Ag/Ab Binding and Elution Buffer Kit is a complete, ready-to-use solution specifically designed for the non-denaturing purification of antibodies using Protein A or Protein G affinity supports. This kit offers an optimized system for preserving antibody and antigen functionality during binding and elution processes, making it ideal for sensitive or labile protein interactions in immunoaffinity chromatography.

Product Description

The kit includes two phosphate-free, pre-filtered buffers:

• Gentle Ag/Ab Binding Buffer (pH 8.0) – a borate-based buffer that facilitates efficient antibody capture by Protein A without the risk of precipitation when used with the Gentle Elution Buffer.
• Gentle Ag/Ab Elution Buffer (pH 6.6) – a high-salt, near-neutral buffer designed to gently dissociate antigen-antibody interactions or release IgG from affinity supports without denaturing or inactivating the antibody or antigen.

Together, these buffers provide a mild alternative to traditional acidic or alkaline elution methods and are especially suitable for purifying antibodies used in functional assays, immunoassays, or downstream enzymatic applications.

Key Features

• Non-denaturing conditions: Maintains native antibody structure and biological activity.
• Phosphate-free: Prevents salt precipitation when combined with elution buffer.
• Ready-to-use: No dilution, pH adjustment, or filtration needed.
• Preserves antigen and antibody function: Suitable for immunoprecipitation and antigen-purification workflows.
• Versatile: Compatible with a wide range of antibody classes and host species when paired with the appropriate immobilized ligand (e.g., Protein A, G, A/G).

Kit Contents

• 100 mL Gentle Ag/Ab Binding Buffer (pH 8.0)
• 100 mL Gentle Ag/Ab Elution Buffer (pH 6.6)

Recommended Applications

• Affinity purification of antibodies (IgG) from serum, ascites, or hybridoma supernatants.
• Antigen isolation using immobilized antibodies.
• Immunoprecipitation and co-immunoprecipitation (co-IP).
• Elution of antigen-antibody complexes from Protein A or G columns.

Storage and Handling

• Store at 4°C
• Shipped at ambient temperature
• Do not use phosphate-containing buffers with this kit to avoid precipitation.

The Pierce Gentle Ag/Ab Binding and Elution Buffer Kit provides a safe and efficient approach to purify functional antibodies and antigens under mild conditions. It is an essential tool for laboratories requiring high-purity antibody preparations without compromising structural or functional integrity—particularly in applications such as ELISA, Western blotting, or therapeutic antibody development.