Invitrogen™ eBioscience™ Integrin beta 7 Monoclonal Antibody (FIB504), FITC, 100 Tests

The Invitrogen™ Integrin beta 7 Monoclonal Antibody (FIB504), conjugated to FITC (Fluorescein Isothiocyanate), is a high-quality reagent designed for detecting Integrin beta 7 in human and mouse samples. This antibody is ideal for flow cytometry applications and provides a bright, photostable fluorescence signal for accurate detection and analysis. Integrin beta 7 plays a crucial role in leukocyte adhesion and migration during immune responses, forming heterodimers with integrin alpha 4 (LPAM-1) or integrin alpha E (HML-1) to mediate interactions with key ligands like VCAM-1, MadCAM-1, and E-cadherin.

Key Features:

1. Target and Specificity:
   • Recognizes Integrin beta 7, a 130 kDa glycoprotein expressed on intraepithelial lymphocytes and T cells adjacent to mucosal epithelium.
   • Forms heterodimers with integrin alpha 4 (CD49d) and alpha E (CD103) for immune cell adhesion.
2. Clone and Conjugate:
   • Clone: FIB504, providing high specificity for integrin beta 7.
   • Conjugate: FITC, offering bright fluorescence for flow cytometry.
3. Validated Applications:
   • Optimized for flow cytometry, enabling robust analysis of integrin beta 7 expression in human and mouse immune cells.
4. Pre-Titrated and Ready-to-Use:
   • Each test requires 5 µL (0.125 µg) of antibody, pre-titrated to simplify experimental workflows.
5. Bright and Stable Fluorescence:
   • FITC provides a high-intensity signal with excitation at 498 nm and emission at 517 nm.

Specifications:

Applications:

1. Flow Cytometry:
   • Detect Integrin beta 7 expression on intraepithelial lymphocytes, mucosal T cells, and other immune cell populations.
   • Study the role of integrin beta 7 in leukocyte adhesion and migration.
2. Immunophenotyping:
   • Characterize lymphocyte populations expressing integrin beta 7 in human and mouse samples.
3. Adhesion Molecule Research:
   • Investigate interactions between integrin beta 7 and its ligands (VCAM-1, MadCAM-1, E-cadherin) in mucosal immunity.
4. Inflammation and Immune Response Studies:
   • Examine the role of integrin beta 7 in leukocyte transmigration during inflammatory responses.
5. Cancer and Infectious Disease Research:
   • Study integrin beta 7 in the context of immune cell homing to inflamed or tumor tissues.

Key Benefits:

1. High Specificity and Sensitivity:
   • The FIB504 clone reliably detects integrin beta 7 with minimal background noise.
2. Bright Fluorescence:
   • FITC provides a robust signal for easy detection in multicolor flow cytometry experiments.
3. Reproducible Results:
   • Validated for flow cytometry, ensuring consistent performance across assays.
4. Ease of Use:
   • Pre-titrated and ready-to-use formulation reduces preparation time.
5. Versatile Research Tool:
   • Suitable for studies in immunology, inflammation, and mucosal immunity.

Usage Recommendations:

1. Sample Preparation:
   • Stain 10⁵–10⁸ cells per test in a final volume of 100 µL using 5 µL of antibody solution.
2. Flow Cytometry:
   • Analyze using a flow cytometer equipped with a blue laser (488 nm).
3. Fixation:
   • Use appropriate fixation buffers to preserve fluorescence after staining.

Storage and Handling:

• Store at 4°C in the dark to protect the FITC fluorochrome.
• Do not freeze, as freezing can degrade the antibody or fluorochrome.

Target Information:

• Integrin beta 7:
  • A key adhesion molecule that associates with integrin alpha 4 (CD49d) or alpha E (CD103) to mediate immune cell migration and adhesion.
  • Facilitates lymphocyte homing to mucosal tissues and leukocyte transmigration during inflammation.
  • Interacts with VCAM-1, MadCAM-1, and E-cadherin.

For Research Use Only:

This product is intended for research purposes only and is not for diagnostic or therapeutic applications.