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The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL00D0) is a high-performance, modular, and upgradeable system that delivers exceptional flexibility for absorbance, fluorescence intensity, and luminescence assays.
Designed to meet the demanding requirements of life science and bioscience research, this multimode reader combines reliable optical precision, automated calibration, and user-friendly software to simplify complex workflows.
Ideal for applications in cell-based assays, enzyme kinetics, nucleic acid quantification, and luminescent reporter assays, the Varioskan LUX provides top and bottom reading modes for maximum assay versatility and performance.
Key Features and Benefits
1. Versatile Detection Technologies
- Combines Absorbance (UV-Vis, 200–1000 nm), Fluorescence Intensity, and Luminescence detection.
- Top and bottom reading optics support a wide range of assays from homogeneous to adherent cell-based formats.
- Dual monochromator system ensures spectral flexibility and precise wavelength selection.
- Compatible with 6–1536 well microplates and μDrop plates for small-volume measurements.
2. Enhanced Sensitivity and Dynamic Range
-
Fluorescence Sensitivity:
- Top: <0.4 fmol fluorescein/well
- Bottom: <4 fmol fluorescein/well
- Luminescence Sensitivity: <7 amol ATP/well (white 384-well plate)
-
Dynamic Range:
- Fluorescence: >6 decades (top), >5.5 decades (bottom)
- Luminescence: >7 decades
- Precision: SD <0.001 Abs or CV <0.5% at 450 nm (0–3 Abs).
3. Intelligent Automation and Workflow Control
- Automatic dynamic range selection optimizes signal intensity per well.
- Smart safety controls identify potential errors before measurement begins.
- Automated calibration and self-diagnostics ensure consistent, long-term performance.
- Spectral scanning and kinetic modes allow for dynamic assay development.
- Integrated orbital shaking and temperature control (ambient +4°C to 45°C) ensure homogeneous reaction conditions.
4. SkanIt™ Software Integration
- Includes Thermo Scientific™ SkanIt™ Software, Research Edition for full control and data analysis.
- License-free installation allows multiple users across different workstations.
- Virtual Pipetting Tool simplifies experiment setup and plate layout configuration.
- Real-time spectral and kinetic data visualization.
- Built-in analysis functions: Blank subtraction, dose-response curves, kinetic modeling, and custom formulas.
- Automatic data saving prevents information loss during interruptions.
- Flexible export options: *.xlsx, *.pdf, *.xml, *.txt, or automatic email export post-run.
5. Certified and Validated Performance
- HTRF™ certified (Cisbio Bioassays) for homogenous time-resolved fluorescence assays.
- DLReady™ validated (Promega Corporation) for luminescent assays.
- Modular design supports future upgrades for AlphaScreen™, TRF, and TR-FRET/hTRF detection.
Technical Specifications
| Parameter | Specification |
|---|---|
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence |
| Reading Options | Top and Bottom |
| Wavelength Range | Absorbance: 200–1000 nm; Fluorescence Excitation: 200–822 nm; Emission: 270–840 nm; Luminescence: 360–670 nm |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | Fluorescence: Top >6 decades, Bottom >5.5 decades; Luminescence >7 decades |
| Sensitivity | FI Top: <0.4 fmol fluorescein/well; FI Bottom: <4 fmol fluorescein/well; Luminescence: <7 amol ATP/well |
| Accuracy (Photometric) | ±2% (200–399 nm, 0–2 Abs); ±1% (400–1000 nm, 0–3 Abs) |
| Precision | SD <0.001 Abs or CV <0.5% at 450 nm |
| Linearity | ±2% (96-well 0–4 Abs; 384-well 0–3 Abs at 450 nm) |
| Spectral Scanning Speed | <2.2 s/well (400–500 nm, 2 nm step) |
| Light Source | Xenon Flash Lamp |
| Detector Type | Photomultiplier Tube (PMT) |
| Incubation Temperature | Ambient +4°C to 45°C |
| Shaking | Orbital |
| Interface | PC Software (SkanIt™ Software) |
| Software Languages | English, French, German, Spanish, Portuguese, Italian, Japanese, Chinese |
| Dimensions (W × D × H) | 53 × 58 × 51 cm (21 × 23 × 20 in) |
| Weight | 56 kg (123 lb) |
| Power Requirements | 100–240 V, 50/60 Hz |
| Plate Types | 6–1536 well plates (Absorbance: up to 384 wells), μDrop plates |
| Software Edition | Research Edition |
Applications
- Nucleic Acid Quantification: DNA, RNA, and protein measurement using absorbance or fluorescence.
- Enzyme Kinetics: Real-time enzyme activity and inhibition studies.
- Cell-Based Assays: Reporter gene analysis, cell viability, and cytotoxicity measurements.
- Luminescence Assays: ATP detection, luciferase assays, and cell signaling studies.
- Fluorescence-Based Studies: FRET, GFP, and fluorescent probe applications.
- Spectral Scanning and Optimization: Excitation/emission profiling for custom assay development.
- Drug Discovery and Screening: Quantitative fluorescence and luminescence-based analysis.
Unique Advantages
- Combines absorbance, fluorescence, and luminescence technologies in one instrument.
- Dual top and bottom reading optics for flexible assay detection.
- Automatic calibration and dynamic range adjustment guarantee reproducibility.
- HTRF™ and DLReady™ certified for validated assay compatibility.
- Upgradeable modular design to expand functionality as research needs evolve.
- Paired with SkanIt™ Software for real-time monitoring, traceability, and reporting.
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL00D0) delivers versatile, high-precision multimode detection for absorbance, fluorescence, and luminescence assays.
It combines superior optical sensitivity, automated workflow control, and advanced software integration, providing researchers with reliable, reproducible, and traceable data.
Designed for molecular biology, biochemical assays, cell-based screening, and drug discovery, the Varioskan LUX empowers laboratories with flexibility, scalability, and unmatched usability for all multimode microplate-based analyses.
Varioskan LUX Multimode Microplate Reader Brochure.pdf
microplate instruments assays accessories.pdf
microplate readers.pdf
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL00D0) is a high-performance, modular, and upgradeable system that delivers exceptional flexibility for absorbance, fluorescence intensity, and luminescence assays.
Designed to meet the demanding requirements of life science and bioscience research, this multimode reader combines reliable optical precision, automated calibration, and user-friendly software to simplify complex workflows.
Ideal for applications in cell-based assays, enzyme kinetics, nucleic acid quantification, and luminescent reporter assays, the Varioskan LUX provides top and bottom reading modes for maximum assay versatility and performance.
Key Features and Benefits
1. Versatile Detection Technologies
- Combines Absorbance (UV-Vis, 200–1000 nm), Fluorescence Intensity, and Luminescence detection.
- Top and bottom reading optics support a wide range of assays from homogeneous to adherent cell-based formats.
- Dual monochromator system ensures spectral flexibility and precise wavelength selection.
- Compatible with 6–1536 well microplates and μDrop plates for small-volume measurements.
2. Enhanced Sensitivity and Dynamic Range
-
Fluorescence Sensitivity:
- Top: <0.4 fmol fluorescein/well
- Bottom: <4 fmol fluorescein/well
- Luminescence Sensitivity: <7 amol ATP/well (white 384-well plate)
-
Dynamic Range:
- Fluorescence: >6 decades (top), >5.5 decades (bottom)
- Luminescence: >7 decades
- Precision: SD <0.001 Abs or CV <0.5% at 450 nm (0–3 Abs).
3. Intelligent Automation and Workflow Control
- Automatic dynamic range selection optimizes signal intensity per well.
- Smart safety controls identify potential errors before measurement begins.
- Automated calibration and self-diagnostics ensure consistent, long-term performance.
- Spectral scanning and kinetic modes allow for dynamic assay development.
- Integrated orbital shaking and temperature control (ambient +4°C to 45°C) ensure homogeneous reaction conditions.
4. SkanIt™ Software Integration
- Includes Thermo Scientific™ SkanIt™ Software, Research Edition for full control and data analysis.
- License-free installation allows multiple users across different workstations.
- Virtual Pipetting Tool simplifies experiment setup and plate layout configuration.
- Real-time spectral and kinetic data visualization.
- Built-in analysis functions: Blank subtraction, dose-response curves, kinetic modeling, and custom formulas.
- Automatic data saving prevents information loss during interruptions.
- Flexible export options: *.xlsx, *.pdf, *.xml, *.txt, or automatic email export post-run.
5. Certified and Validated Performance
- HTRF™ certified (Cisbio Bioassays) for homogenous time-resolved fluorescence assays.
- DLReady™ validated (Promega Corporation) for luminescent assays.
- Modular design supports future upgrades for AlphaScreen™, TRF, and TR-FRET/hTRF detection.
Technical Specifications
| Parameter | Specification |
|---|---|
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence |
| Reading Options | Top and Bottom |
| Wavelength Range | Absorbance: 200–1000 nm; Fluorescence Excitation: 200–822 nm; Emission: 270–840 nm; Luminescence: 360–670 nm |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | Fluorescence: Top >6 decades, Bottom >5.5 decades; Luminescence >7 decades |
| Sensitivity | FI Top: <0.4 fmol fluorescein/well; FI Bottom: <4 fmol fluorescein/well; Luminescence: <7 amol ATP/well |
| Accuracy (Photometric) | ±2% (200–399 nm, 0–2 Abs); ±1% (400–1000 nm, 0–3 Abs) |
| Precision | SD <0.001 Abs or CV <0.5% at 450 nm |
| Linearity | ±2% (96-well 0–4 Abs; 384-well 0–3 Abs at 450 nm) |
| Spectral Scanning Speed | <2.2 s/well (400–500 nm, 2 nm step) |
| Light Source | Xenon Flash Lamp |
| Detector Type | Photomultiplier Tube (PMT) |
| Incubation Temperature | Ambient +4°C to 45°C |
| Shaking | Orbital |
| Interface | PC Software (SkanIt™ Software) |
| Software Languages | English, French, German, Spanish, Portuguese, Italian, Japanese, Chinese |
| Dimensions (W × D × H) | 53 × 58 × 51 cm (21 × 23 × 20 in) |
| Weight | 56 kg (123 lb) |
| Power Requirements | 100–240 V, 50/60 Hz |
| Plate Types | 6–1536 well plates (Absorbance: up to 384 wells), μDrop plates |
| Software Edition | Research Edition |
Applications
- Nucleic Acid Quantification: DNA, RNA, and protein measurement using absorbance or fluorescence.
- Enzyme Kinetics: Real-time enzyme activity and inhibition studies.
- Cell-Based Assays: Reporter gene analysis, cell viability, and cytotoxicity measurements.
- Luminescence Assays: ATP detection, luciferase assays, and cell signaling studies.
- Fluorescence-Based Studies: FRET, GFP, and fluorescent probe applications.
- Spectral Scanning and Optimization: Excitation/emission profiling for custom assay development.
- Drug Discovery and Screening: Quantitative fluorescence and luminescence-based analysis.
Unique Advantages
- Combines absorbance, fluorescence, and luminescence technologies in one instrument.
- Dual top and bottom reading optics for flexible assay detection.
- Automatic calibration and dynamic range adjustment guarantee reproducibility.
- HTRF™ and DLReady™ certified for validated assay compatibility.
- Upgradeable modular design to expand functionality as research needs evolve.
- Paired with SkanIt™ Software for real-time monitoring, traceability, and reporting.
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL00D0) delivers versatile, high-precision multimode detection for absorbance, fluorescence, and luminescence assays.
It combines superior optical sensitivity, automated workflow control, and advanced software integration, providing researchers with reliable, reproducible, and traceable data.
Designed for molecular biology, biochemical assays, cell-based screening, and drug discovery, the Varioskan LUX empowers laboratories with flexibility, scalability, and unmatched usability for all multimode microplate-based analyses.
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