Thermo Scientific™ Bromophenol blue

Bromophenol Blue (BPB) is a pH indicator and tracking dye commonly used in biochemical assays, gel electrophoresis, and acid-base titrations. Supplied in ACS reagent-grade purity by Thermo Fisher Scientific, this compound exhibits a distinct visual transition from yellow to blue between pH 3.0 and 4.6. Its strong colorimetric response and compatibility with aqueous systems make it a staple reagent in protein and nucleic acid research.

Bromophenol Blue is often used to monitor sample migration during electrophoresis and can serve as a visual endpoint marker in pH-sensitive reactions and tests.

Key Features

• ACS-grade reagent for analytical and biochemical applications
• pH transition interval: pH 3.0 (yellow) to pH 4.6 (blue)
• Color varies from pink-orange to red to purple in solid form
• High-purity powder with confirmed infrared spectrum and clarity test
• Stable at ambient temperatures
• Excellent solubility in aqueous and some polar solvents

Chemical & Physical Properties

Packaging Options

Applications

• pH indicator in analytical titrations
• Tracking dye in agarose and polyacrylamide gel electrophoresis
• Sample loading dye in SDS-PAGE and nucleic acid gels
• Indicator in protein quantification (e.g., Bradford assay)
• Colorimetric detection in acid-base reaction monitoring
• pH control and buffering system validation

Thermo Fisher Scientific Bromophenol Blue ACS offers a high-purity, reliable reagent for biochemical research, electrophoresis, and acid-base analytical methods. With a sharp visual pH transition and excellent stability, it serves as a trusted indicator and migration marker for routine laboratory and research