Thermo Scientific™ Bromelain Powder from Stem

Cysteine Endopeptidase for Protein Hydrolysis, Anti-inflammatory Studies, and Proteomic Applications

Thermo Scientific™ Bromelain Powder is a purified proteolytic enzyme derived from pineapple stem, classified as a cysteine endopeptidase with broad substrate specificity. Known for its strong proteolytic activity under mildly acidic conditions, bromelain is widely used in biochemical assays, inflammation studies, protein hydrolysis, and enzyme-substrate interaction research.

This enzyme preparation supports diverse experimental workflows, from micellar phase system studies and chromogenic protease assays to SDS-PAGE controls and anti-inflammatory screening. It is also employed in enzymatic hydrolysis of complex biological substrates, including pollen and peptides containing opioid-like sequences.

Key Features & Benefits

• Cysteine Endopeptidase Activity – Broad proteolytic specificity for protein cleavage
• Stem-Derived Enzyme – Isolated from pineapple stem for consistent activity and purity
• Functional Versatility – Used in cell proliferation assays, SDS-PAGE, analgesic studies, and substrate hydrolysis
• Standardized Enzyme Activity – Defined in μmol p-nitrophenol released per minute for precise assay design
• Ready for Buffer Suspension – Forms stable suspensions in acetate buffer (pH 4.5) at 1 mg/mL

Applications

• Protease Assays – Assayed with chromogenic substrates like pGlu-Phe-Leu-p-nitroanilide
• Enzymatic Hydrolysis – Degrades proteins in complex substrates such as pollen or peptide derivatives
• Anti-inflammatory Research – Used to study inhibition of proinflammatory prostaglandin biosynthesis
• Clotting Studies – Investigated for its ability to reduce clotting efficiency
• Micellar System Evaluation – Used in aqueous micellar two-phase systems with ionic liquids
• Biochemical Controls – Acts as a positive control in SDS-PAGE and cell proliferation assays

Biochemical/Physiological Actions

• Class: Cysteine protease (endopeptidase)
• Source: Pineapple stem
• Specificity: Broad; cleaves a variety of peptide bonds
• Inhibitory Role: May inhibit proinflammatory prostaglandins
• Blood Effect: Can reduce clotting efficiency

Enzyme Activity (Unit Definition)

• One unit liberates 1.0 µmol of p-nitrophenol from N-α-CBZ-L-lysine p-nitrophenyl ester per minute
• Assay Conditions: pH 4.6 at 25 °C
• Conversion: 1 old unit at pH 4.5 / 45 °C ≈ 1.7 new units at pH 4.6 / 25 °C

Preparation & Handling

Thermo Scientific™ Bromelain Powder from Stem is a high-performance cysteine protease suited for analytical proteomics, protein degradation studies, and inflammation research. Its broad substrate range and defined enzymatic activity make it a reliable tool for researchers working across biochemistry, immunology, enzymology, and cellular biology.