Atom Sci. Orange G Stain Commission Certified C.I. 16230

Atom Scientific Orange G Stain is a red-orange powder commonly used in cytological and histological staining protocols. Its primary application is in the OG6 Papanicolaou (Pap) stain, where it plays a key role in differentiating keratinized cells. The C.I. 16230 Commission Certified grade ensures high quality and reliable performance for routine and specialized staining procedures.

Key Features

1. Certified Quality

• Commission Certified (C.I. 16230):
  • Ensures adherence to international standards.
  • Provides reliable and consistent staining results.

2. Physical Properties

• Appearance: Red-orange powder.
• Solubility: Readily dissolves in water or ethanol, forming a stable solution.
• Chemical Class: Acidic azo dye.
• Molecular Formula: C16H10N2Na2O7S2.

3. Primary Application

• OG6 Papanicolaou Stain:
  • Highlights keratinized cells with shades of orange.
  • Used extensively in cytology for cervical smear screening and histological tissue differentiation.

4. Versatility

• Compatible with other staining protocols that require a cytoplasmic or connective tissue-specific acidic dye.

Applications

1. Cytology:
   • Integral part of the Papanicolaou (Pap) stain for cytological evaluation of exfoliated cells.
2. Histology:
   • Demonstrates keratinized cells in tissue sections.
   • Used in staining protocols for identifying keratinized layers in epithelial tissues.
3. Research and Diagnostics:
   • Widely used in research and clinical labs for cellular morphology studies.

Advantages

1. Reliable Results:
   • Certified composition ensures consistent performance for diagnostic and research purposes.
2. High Contrast Staining:
   • Enhances visualization of keratinized cells, aiding in clear differentiation.
3. Compatibility:
   • Integrates seamlessly into manual and automated staining workflows.
4. Ease of Use:
   • Soluble in water and ethanol for convenient preparation and application.

Preparation and Usage

Preparation:

1. Dissolve Orange G powder in distilled water or ethanol to prepare a working solution (typically 1% concentration).
2. Adjust the pH as required for specific staining protocols.

Staining Protocol:

1. Use as part of the OG6 Papanicolaou stain.
2. After nuclear staining, immerse slides in Orange G solution for cytoplasmic staining.
3. Rinse slides to remove excess dye and proceed with further staining or dehydration steps.

Specifications

Storage and Handling

• Store at room temperature in a dry, sealed container.
• Protect from light and moisture to maintain stability.
• Handle with appropriate personal protective equipment (PPE).

Safety Precautions

• Avoid inhalation, ingestion, or direct contact with the skin and eyes.
• Use in a well-ventilated laboratory environment.
• Dispose of waste according to local regulations.
• Refer to the safety data sheet (SDS) for comprehensive hazard details.

The Atom Scientific Orange G Stain (Commission Certified C.I. 16230) is an essential reagent for cytology and histology laboratories, providing reliable and high-quality staining for keratinized cells. Its consistent performance and compatibility with the OG6 Papanicolaou stain make it a trusted choice for both clinical diagnostics and research applications.