Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit

The Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit is a high-performance real-time PCR solution designed for sensitive and accurate gene expression analysis directly from RNA templates. This optimized one-step RT-qPCR system integrates reverse transcription and quantitative PCR amplification into a single reaction, reducing handling time, minimizing contamination risk, and improving reproducibility.

Featuring a proprietary formulation that reduces primer-dimer formation and non-specific amplification, the kit delivers highly reliable quantification of low-abundance RNA targets. It combines ArrayScript™ UP Reverse Transcriptase for efficient cDNA synthesis with AmpliTaq™ Gold DNA Polymerase UP, providing hot-start PCR specificity and robust amplification performance.

The inclusion of SYBR™ Green dye enables sensitive fluorescence-based detection, while the built-in ROX™ passive reference dye ensures precise normalization across Applied Biosystems™ real-time PCR instruments.

This kit is ideal for gene expression studies, RNA quantification, and molecular biology research workflows requiring fast, sensitive, and reproducible RT-qPCR results.

Key Features

High Sensitivity and Specificity

• Detects low-abundance RNA targets with high precision
• Optimized formulation reduces primer-dimer formation
• Minimizes false positive results

Efficient One-Step RT-qPCR Workflow

• Reverse transcription and PCR amplification in a single tube
• Reduces workflow time and contamination risk
• Simplifies experimental setup

Advanced Enzyme System

• ArrayScript™ UP Reverse Transcriptase
  • Produces high yields of full-length cDNA
  • Efficient transcription of challenging RNA templates
• AmpliTaq™ Gold DNA Polymerase UP
  • Hot-start enzyme minimizes non-specific amplification
  • Improves accuracy and reproducibility

Built-in Precision Control

• Integrated ROX™ passive reference dye
• Normalizes fluorescence variation
• Improves quantification accuracy across Applied Biosystems instruments

Optimized Master Mix Formulation

• Includes SYBR™ Green dye for sensitive detection
• Contains RNase inhibitor for RNA protection
• Ready-to-use format reduces preparation time

Kit Contents and Storage

Technical Specifications

Compatible Real-Time PCR Instruments

Fully compatible with Applied Biosystems™ real-time PCR platforms, including:

• QuantStudio™ 3, 5, 6 Flex, 7, and 12K Flex
• StepOne™ and StepOnePlus™ Systems
• 7500 and 7500 Fast Real-Time PCR Systems
• 7900HT Fast Real-Time PCR System
• ViiA™ 7 Real-Time PCR System

Applications

The Power SYBR™ Green RNA-to-CT™ 1-Step Kit is ideal for:

• Gene expression analysis
• RNA quantification
• mRNA expression profiling
• Pathogen detection research
• Biomarker discovery
• Molecular biology research
• Validation of sequencing results
• Quantitative transcript analysis

Workflow Integration

RNA Template → Reverse Transcription → cDNA Amplification → SYBR Green Detection → Real-Time Quantification

Advantages Over Two-Step RT-PCR Methods

The Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit provides a highly sensitive, reliable, and streamlined solution for RNA-based gene expression analysis. With integrated reverse transcription and amplification, advanced enzyme technology, and precise SYBR Green detection, this kit delivers accurate and reproducible RT-qPCR results while simplifying laboratory workflows.