Human Strongyloides IgG/IgM ELISA kit

The Strongyloides IgG/IgM ELISA Kit (ab247203) is a qualitative sandwich ELISA assay designed for the detection of Strongyloides IgG and IgM antibodies in human serum, citrate plasma, and heparin plasma samples. This kit is ideal for research into Strongyloides infections and immune responses.

Kit Features

1. Qualitative Detection:
   • Measures Strongyloides IgG/IgM levels in human samples.
2. Wide Sample Compatibility:
   • Validated for serum, citrate plasma, and heparin plasma.
3. Complete Kit:
   • Includes all necessary components for a straightforward and reliable assay.

Kit Components

1. Strongyloides Coated Microplate (1 Unit)
2. Strongyloides Cut-off Control (3 mL)
3. Strongyloides Negative Control (2 mL)
4. Strongyloides Positive Control (2 mL)
5. 20X Washing Solution (50 mL)
6. Sample Diluent (100 mL)
7. TMB Substrate Solution (15 mL)
8. Stop Solution (15 mL)
9. anti-human IgG/IgM HRP Conjugate (20 mL)
10. Cover Foil (1 Unit)
11. Protocol Booklet

Applications

• Strongyloides Research:
  • Study immune responses to Strongyloides stercoralis infection.
• Serological Testing:
  • Assess presence of IgG and IgM antibodies in Strongyloides infections.
• Infectious Disease Studies:
  • Investigate prevalence and immune responses in Strongyloides endemic regions.
• Clinical Diagnostics Research:
  • Support diagnostic evaluations for Strongyloides-related infections.

Storage and Stability

• Store all kit components as per the manufacturer's guidelines, typically at 2–8°C.
• Avoid repeated freeze-thaw cycles to ensure reagent integrity.

Assay Protocol Overview

1. Sample Preparation:
   • Dilute samples using the provided sample diluent.
2. Incubation:
   • Add samples and controls to the coated microplate wells and incubate.
3. Washing:
   • Wash wells using the provided 20X washing solution.
4. Detection:
   • Add HRP-conjugated anti-human IgG/IgM and incubate.
5. Substrate Reaction:
   • Add TMB substrate and incubate to develop color.
6. Stop Reaction and Measurement:
   • Add stop solution and measure optical density (OD) at 450 nm.

Advantages

1. Comprehensive Controls:
   • Includes positive, negative, and cutoff controls for accurate and reliable results.
2. Ease of Use:
   • Pre-coated plates and ready-to-use reagents simplify assay setup.
3. Reproducibility:
   • Consistent performance for robust data interpretation.

The Abcam Human Strongyloides IgG/IgM ELISA Kit (ab247203) is an essential tool for researchers studying Strongyloides infections and associated immune responses. Its qualitative detection, user-friendly protocol, and reliable components make it an invaluable asset for serological and infectious disease research.