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The Gibco™ B-27™ Plus Neuronal Culture System represents the next generation of serum-free neuronal cell culture media, offering unmatched in vitro neuron survival and functionality.
This optimized two-part system includes Gibco™ B-27™ Plus Supplement (50X) and Gibco™ Neurobasal™ Plus Medium, providing a fully integrated and high-performance solution for the culture, maturation, and maintenance of primary rodent and human stem cell–derived neurons.
Building on the legacy of the classic B-27™ Supplement and Neurobasal™ Medium, this improved system features refined formulation, upgraded raw material controls, and stringent cGMP manufacturing to ensure the highest reproducibility and cell culture performance.
Key Features
- Next-generation neuronal system: Combines B-27™ Plus Supplement and Neurobasal™ Plus Medium for superior results.
- Superior neuron survival: Achieves ≥50% higher viability of primary rat neurons versus traditional B-27™/Neurobasal™ combinations.
- Enhanced electrophysiological activity: Promotes consistent, synchronized, and stable neuronal firing after 10 days in vitro.
- Improved maturation: Accelerates neurite outgrowth and increases synaptic-positive puncta formation.
- Broad compatibility: Supports primary rat, mouse, and human stem cell–derived neurons.
- High-quality manufacturing: Produced under cGMP and ISO 13485-certified conditions.
- Research Use Only (RUO): Not for use in diagnostic procedures.
System Components
| Component | Description | Storage Conditions |
|---|---|---|
| B-27™ Plus Supplement (50X) | 10 mL, serum-free neuronal supplement | −5 to −20 °C; protect from light |
| Neurobasal™ Plus Medium | 500 mL, basal neuronal medium | 2–8 °C; protect from light |
Specifications
| Parameter | Specification |
|---|---|
| Cell Type | Neural cells (primary and stem cell–derived) |
| Product Line | B-27™, Neurobasal™ |
| Product Type | Neuronal culture system |
| Classification | Serum-free |
| Culture Type | Mammalian cell culture |
| Form | Liquid |
| Shelf Life | 12 months |
| Shipping Conditions | Ambient |
| Quantity | 1 System |
| Intended Use | Research Use Only (RUO) |
Applications
- Primary neuronal culture: Rat, mouse, and human neurons.
- Stem cell research: Differentiation and maturation of PSC- or iPSC-derived neurons.
- Neurobiology research: Studies on neurite outgrowth, synaptogenesis, and functional activity.
- Electrophysiology: For high-fidelity neuronal firing and network activity assays.
- Drug discovery and neurotoxicity testing.
The Gibco™ B-27™ Plus Neuronal Culture System delivers superior neuronal survival, maturation, and electrophysiological performance, redefining the standard for serum-free neuronal culture.
Combining B-27™ Plus Supplement (50X) and Neurobasal™ Plus Medium, this integrated solution provides the highest consistency, purity, and functional maturity for both rodent and human stem cell–derived neurons.
Manufactured under cGMP and ISO 13485 standards, it is the most advanced neuronal culture platform for modern neurobiology research.
Brochure: B-27 Plus Neuronal Culture System
User Guide: B-27 Plus Neuronal Culture System (PSC-Derived Neuron Applications)
User Guide: B-27 Plus Neuronal Culture System (Primary Neuron Applications)
The Gibco™ B-27™ Plus Neuronal Culture System represents the next generation of serum-free neuronal cell culture media, offering unmatched in vitro neuron survival and functionality.
This optimized two-part system includes Gibco™ B-27™ Plus Supplement (50X) and Gibco™ Neurobasal™ Plus Medium, providing a fully integrated and high-performance solution for the culture, maturation, and maintenance of primary rodent and human stem cell–derived neurons.
Building on the legacy of the classic B-27™ Supplement and Neurobasal™ Medium, this improved system features refined formulation, upgraded raw material controls, and stringent cGMP manufacturing to ensure the highest reproducibility and cell culture performance.
Key Features
- Next-generation neuronal system: Combines B-27™ Plus Supplement and Neurobasal™ Plus Medium for superior results.
- Superior neuron survival: Achieves ≥50% higher viability of primary rat neurons versus traditional B-27™/Neurobasal™ combinations.
- Enhanced electrophysiological activity: Promotes consistent, synchronized, and stable neuronal firing after 10 days in vitro.
- Improved maturation: Accelerates neurite outgrowth and increases synaptic-positive puncta formation.
- Broad compatibility: Supports primary rat, mouse, and human stem cell–derived neurons.
- High-quality manufacturing: Produced under cGMP and ISO 13485-certified conditions.
- Research Use Only (RUO): Not for use in diagnostic procedures.
System Components
| Component | Description | Storage Conditions |
|---|---|---|
| B-27™ Plus Supplement (50X) | 10 mL, serum-free neuronal supplement | −5 to −20 °C; protect from light |
| Neurobasal™ Plus Medium | 500 mL, basal neuronal medium | 2–8 °C; protect from light |
Specifications
| Parameter | Specification |
|---|---|
| Cell Type | Neural cells (primary and stem cell–derived) |
| Product Line | B-27™, Neurobasal™ |
| Product Type | Neuronal culture system |
| Classification | Serum-free |
| Culture Type | Mammalian cell culture |
| Form | Liquid |
| Shelf Life | 12 months |
| Shipping Conditions | Ambient |
| Quantity | 1 System |
| Intended Use | Research Use Only (RUO) |
Applications
- Primary neuronal culture: Rat, mouse, and human neurons.
- Stem cell research: Differentiation and maturation of PSC- or iPSC-derived neurons.
- Neurobiology research: Studies on neurite outgrowth, synaptogenesis, and functional activity.
- Electrophysiology: For high-fidelity neuronal firing and network activity assays.
- Drug discovery and neurotoxicity testing.
The Gibco™ B-27™ Plus Neuronal Culture System delivers superior neuronal survival, maturation, and electrophysiological performance, redefining the standard for serum-free neuronal culture.
Combining B-27™ Plus Supplement (50X) and Neurobasal™ Plus Medium, this integrated solution provides the highest consistency, purity, and functional maturity for both rodent and human stem cell–derived neurons.
Manufactured under cGMP and ISO 13485 standards, it is the most advanced neuronal culture platform for modern neurobiology research.
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