Applied Biosystems™ QuantStudio™ 3 Real-Time PCR System, 96-well, 0.2 mL, laptop

The QuantStudio™ 3 Real-Time PCR System offers affordability, ease of use, and reliable performance for researchers of all experience levels. It combines proven OptiFlex technology, cloud connectivity, and intuitive software to deliver high-quality qPCR data for a wide range of genomic applications.

Key Features and Benefits

1. Proven OptiFlex Technology:
   • Four coupled excitation and emission filter channels with white LED.
   • Delivers improved well-to-well and instrument-to-instrument data accuracy.
   • Offers enhanced sensitivity to detect small target quantity changes (as small as 1.5-fold).
2. VeriFlex Temperature Control:
   • Features three independent temperature zones for precise optimization of PCR conditions.
3. Interactive Touchscreen:
   • Simplified instrument software for easy navigation.
   • Pre-optimized protocol templates for quick experiment setup.
4. Cloud-Enabled Connectivity with iConnect:
   • Upload, store, and analyze data securely from anywhere with internet access.
   • Flexible web browser-based qPCR analysis modules for quick and efficient data handling.
5. Software Options:
   • Design and Analysis Cloud Software:
     • Accessible via web browser (no software installation required).
     • Provides real-time data access, no licenses needed.
   • Design and Analysis Desktop Software:
     • Traditional PC-based system configuration for localized use.
     • No additional license costs.
6. Compact and User-Friendly:
   • Factory-calibrated and delivered in easy-to-use packaging for quick installation.
   • Compact design allows placement in limited bench spaces.
7. High Application Versatility:
   • Supports diverse genomic applications including:
     • Gene expression
     • Genetic variation
     • Gene regulation
     • SNP genotyping
     • MicroRNA profiling

Technical Specifications

Applications

1. Gene Expression Analysis: Quantify mRNA and study gene regulation.
2. Genetic Variation Studies: SNP genotyping and allele discrimination.
3. MicroRNA Profiling: Detect and analyze microRNA expression levels.
4. High-Resolution Melt Analysis (HRM): Study epigenetic changes and mutation detection.
5. Pathogen Detection: Identify and quantify pathogen DNA/RNA in samples.

Included Components

• QuantStudio 3 Real-Time PCR System
• Laptop (pre-configured for system control)
• Power cords and accessories

Why Choose the QuantStudio™ 3 System?

• Affordable and Compact: Ideal for labs with budget and space constraints.
• Flexible and Accessible: Cloud-enabled for remote data access and management.
• User-Friendly: Simplified workflows and pre-optimized protocols for seamless operation.
• Proven Technology: Trusted OptiFlex and VeriFlex technologies ensure accuracy and reliability.
• Versatile Applications: Suitable for a wide range of genomic studies.

When selecting a real-time PCR system, it's essential to compare various models to identify the one that best fits your laboratory's needs. Below is a detailed comparison of the Applied Biosystems™ QuantStudio™ 3 Real-Time PCR System with its counterparts, the QuantStudio™ 1 and QuantStudio™ 5, as well as comparable models from other leading manufacturers.

Comparison Table:

Key Considerations:

• Temperature Control: The QuantStudio™ 3 and 5 models feature VeriFlex technology, providing multiple independent temperature zones for precise PCR optimization. In contrast, the QuantStudio™ 1 offers a single temperature zone. Models like the Bio-Rad CFX96 Touch and Roche LightCycler 96 offer gradient capabilities for temperature optimization.
• Optical Channels and Multiplexing: The QuantStudio™ 5 offers up to 6 optical channels (in the 96-well configuration), enabling higher multiplexing capabilities compared to the QuantStudio™ 3 and 1, which offer up to 4 channels. This allows for the detection of more targets per run.
• Connectivity and Software: The QuantStudio™ 3 and 5 systems are cloud-enabled, allowing for remote data access and analysis via Thermo Fisher's cloud platform. This feature is particularly beneficial for collaborative environments and data management.
• Applications and Flexibility: The QuantStudio™ 5 system supports both 96-well and 384-well formats, offering greater flexibility for varying throughput needs. 
• Calibration and Maintenance: Factory calibration in the QuantStudio™ systems ensures consistent performance and reduces the need for user intervention. Some competitor models require user calibration, which may necessitate additional maintenance.

When choosing a real-time PCR system, consider factors such as throughput requirements, multiplexing needs, connectivity preferences, and budget constraints. The QuantStudio™ series offers a range of options to accommodate different laboratory needs, with the QuantStudio™ 3 providing a balance of performance and affordability, and the QuantStudio™ 5 offering enhanced features for more demanding applications.